New urinary matabolites formed from ring-oxidized metabolic intermediates of styrene

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F10%3A00022959" target="_blank" >RIV/60461373:22310/10:00022959 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New urinary matabolites formed from ring-oxidized metabolic intermediates of styrene

Popis výsledku v původním jazyce

The urine from mice exposed to styrene vapors (600 and 1200 mg/m3, 6h) was analyzed for ring-oxidized metabolites of styrene. To facilitate the identification of metabolites in urine, following potential metabolites were prepared: 2-, 3-, and 4-vinylphenol (2-, 3- and 4-VP), 4-vinylpyrocatechol, and 2-, 3- and 4-vinylphenylmercapturic acid (2-, 3- and 4-VPMA). For the analysis of vinylphenols beta-glucuronidase-treated urine was extracted and derivatized with acetanhydride/ triethylamine before the injection into GC/MS. Three isomers, 2-, 3- and 4-VP, were found in the exposed urine using authentic standards. Additionally, three novel minor urinary metabolites, arylmercapturic acids 2-, 3- and 4-VPMA, were identified by LC-ESI-MS2 by comparison with authentic standards. Excretion of the most abundant isomer, 4-VPMA, amounted to 535 +/- 47 nmol/kg and 984 +/- 78 nmol/kg, representing approx. 0.047 and 0.043 % of the absorbed dose for the exposure levels of 600 and 1200 mg/m3, respective

Název v anglickém jazyce

New urinary matabolites formed from ring-oxidized metabolic intermediates of styrene

Popis výsledku anglicky

The urine from mice exposed to styrene vapors (600 and 1200 mg/m3, 6h) was analyzed for ring-oxidized metabolites of styrene. To facilitate the identification of metabolites in urine, following potential metabolites were prepared: 2-, 3-, and 4-vinylphenol (2-, 3- and 4-VP), 4-vinylpyrocatechol, and 2-, 3- and 4-vinylphenylmercapturic acid (2-, 3- and 4-VPMA). For the analysis of vinylphenols beta-glucuronidase-treated urine was extracted and derivatized with acetanhydride/ triethylamine before the injection into GC/MS. Three isomers, 2-, 3- and 4-VP, were found in the exposed urine using authentic standards. Additionally, three novel minor urinary metabolites, arylmercapturic acids 2-, 3- and 4-VPMA, were identified by LC-ESI-MS2 by comparison with authentic standards. Excretion of the most abundant isomer, 4-VPMA, amounted to 535 +/- 47 nmol/kg and 984 +/- 78 nmol/kg, representing approx. 0.047 and 0.043 % of the absorbed dose for the exposure levels of 600 and 1200 mg/m3, respective

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/2B08051" target="_blank" >2B08051: Nová strategie biologického monitorování exposice mutagenům a karcinogenům</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chem. Res. Toxicol.

ISSN

0893-228X

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000273618500029

EID výsledku v databázi Scopus

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