Amine functionalized carbon nanoparticles grafted biopolymer for cell adhesion and proliferation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F15%3A43900454" target="_blank" >RIV/60461373:22310/15:43900454 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Amine functionalized carbon nanoparticles grafted biopolymer for cell adhesion and proliferation

Popis výsledku v původním jazyce

Various carbon nanostructures are widely researched for use in number of medical applications. We evaluated the surface properties and cell-substrate interactions of three types of amine functionalized carbon nanoparticles (CNPs) grafted on biopolymer film. Poly-L-lactic acid, in form of polymer film, was treated in an inert argon plasma discharge and, subsequently, grafted with functionalized carbon nanoparticles. Selected samples were thermally stressed during or following the grafting procedure. The surface properties were studied using multiple methods, including goniometry, atomic force microscopy and X-ray photoelectron spectroscopy. Cell-substrate interactions were determined in vitro by studying adhesion, proliferation and viability of vascular smooth muscle cells (VSMCs) from the aorta of a rat. Cell-substrate interactions on pristine and modified substrates were compared to standard tissue culture polystyrene (TCPS). Our results show that CNPs affect surface morphology and wettability and therefore adhesion, proliferation and viability of cultured cells.

Název v anglickém jazyce

Amine functionalized carbon nanoparticles grafted biopolymer for cell adhesion and proliferation

Popis výsledku anglicky

Various carbon nanostructures are widely researched for use in number of medical applications. We evaluated the surface properties and cell-substrate interactions of three types of amine functionalized carbon nanoparticles (CNPs) grafted on biopolymer film. Poly-L-lactic acid, in form of polymer film, was treated in an inert argon plasma discharge and, subsequently, grafted with functionalized carbon nanoparticles. Selected samples were thermally stressed during or following the grafting procedure. The surface properties were studied using multiple methods, including goniometry, atomic force microscopy and X-ray photoelectron spectroscopy. Cell-substrate interactions were determined in vitro by studying adhesion, proliferation and viability of vascular smooth muscle cells (VSMCs) from the aorta of a rat. Cell-substrate interactions on pristine and modified substrates were compared to standard tissue culture polystyrene (TCPS). Our results show that CNPs affect surface morphology and wettability and therefore adhesion, proliferation and viability of cultured cells.

Druh

O - Ostatní výsledky

CEP obor

JJ - Ostatní materiály

OECD FORD obor

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Projekt

<a href="/cs/project/NV15-33018A" target="_blank" >NV15-33018A: Využití kmenových buněk tukové tkáně získané liposukcí v tkáňovém inženýrství</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů