High power plasma as an efficient tool for polymethylpentene cytocompatibility enhancement

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F16%3A43902212" target="_blank" >RIV/60461373:22310/16:43902212 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389005:_____/16:00463672

Výsledek na webu

<a href="http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C6RA14949A#!divAbstract" target="_blank" >http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C6RA14949A#!divAbstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c6ra14949a" target="_blank" >10.1039/c6ra14949a</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High power plasma as an efficient tool for polymethylpentene cytocompatibility enhancement

Popis výsledku v původním jazyce

High power plasma was successfully used as an efficient and inexpensive tool for polymethylpentene (PMP) cytocompatibility enhancement. Proper surface roughness and morphology as well as the surface chemistry of a material are essential factors for successful utilization of a substrate intended for tissue engineering applications. Plasma treatment is an inexpensive, simple and highly effective method for optimization of surface properties, as a result of which enhancement of cell adhesion, growth and migration occurs. Oxygen and argon plasma treatments were applied to activate the PMP surface. The research was focused on the investigation of the changed properties. The AFM and FIB-SEM study demonstrated that plasma treatments of PMP induced structured surfaces which depended on the applied plasma setting. Measurement of the goniometry, and chemical changes (functional groups by FTIR, element concentration by XPS and RBS/ERDA) was included. Experiments on treated substrates with mouse fibroblasts (NIH 3T3) have shown a significant increase in cell adhesion and proliferation on treated PMP substrates when compared to the untreated polymer. Similarly, cell morphology and adhesion sites were carefully examined using fluorescence and scanning electron microscopy; results of both techniques confirmed the suitability of treated PMP samples for cell cultivation. This study demonstrates how to easily improve cytocompatibility of a very inert and resistant polymer for tissue engineering applications.

Název v anglickém jazyce

High power plasma as an efficient tool for polymethylpentene cytocompatibility enhancement

Popis výsledku anglicky

High power plasma was successfully used as an efficient and inexpensive tool for polymethylpentene (PMP) cytocompatibility enhancement. Proper surface roughness and morphology as well as the surface chemistry of a material are essential factors for successful utilization of a substrate intended for tissue engineering applications. Plasma treatment is an inexpensive, simple and highly effective method for optimization of surface properties, as a result of which enhancement of cell adhesion, growth and migration occurs. Oxygen and argon plasma treatments were applied to activate the PMP surface. The research was focused on the investigation of the changed properties. The AFM and FIB-SEM study demonstrated that plasma treatments of PMP induced structured surfaces which depended on the applied plasma setting. Measurement of the goniometry, and chemical changes (functional groups by FTIR, element concentration by XPS and RBS/ERDA) was included. Experiments on treated substrates with mouse fibroblasts (NIH 3T3) have shown a significant increase in cell adhesion and proliferation on treated PMP substrates when compared to the untreated polymer. Similarly, cell morphology and adhesion sites were carefully examined using fluorescence and scanning electron microscopy; results of both techniques confirmed the suitability of treated PMP samples for cell cultivation. This study demonstrates how to easily improve cytocompatibility of a very inert and resistant polymer for tissue engineering applications.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JJ - Ostatní materiály

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RSC Advances

ISSN

2046-2069

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

79

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

76000-76010

Kód UT WoS článku

000381513800117

EID výsledku v databázi Scopus

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