Effect of polymer modification on the cytocompatibility of human and rat cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F17%3A43915138" target="_blank" >RIV/60461373:22310/17:43915138 - isvavai.cz</a>

Výsledek na webu

<a href="https://waset.org/Publication/effect-of-the-polymer-modification-on-the-cytocompatibility-of-human-and-rat-cells/10007722" target="_blank" >https://waset.org/Publication/effect-of-the-polymer-modification-on-the-cytocompatibility-of-human-and-rat-cells/10007722</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of polymer modification on the cytocompatibility of human and rat cells

Popis výsledku v původním jazyce

Tissue engineering includes use of a combination of cells, engineering and materials methods for the formation of new viable tissue for a medical purpose. Scaffolds represent important components for tissue engineering. For some applications, the great advantage of several polymeric materials is their cytocompatibility and susceptibility to biodegradation. Poly(L-lactic acid) (PLLA) is a fully biodegradable polymer which further degrades into H2O and CO2. The crucial factor for successful application of tissue replacements is the interaction of a graft with its surrounding tissue. This interaction involves cell adhesion, adsorption of extracellular molecules and support of cell proliferation. In this experiment, the effect of the surface modification of biodegradable polymer (performed by plasma treatment) on the various cell types was studied. The surface parameters and changes of the physicochemical properties of modified PLLA substrates were studied by different methods. Surface wettability was determined by goniometry, surface morphology and roughness study was performed with atomic force microscopy and chemical composition was determined using photoelectron spectroscopy. The physicochemical properties were studied in relation to cytocompatibility of human osteoblast (MG 63 cells), rat vascular smooth muscle cells (VSMC) and human stem cells (ASC) of the adipose tissue in vitro. A fluorescence microscopy was chosen to study and compare cell-material interaction. Important parameters of the cytocompatibility like adhesion, proliferation, viability, shape, spreading of the above-mentioned cells were evaluated. It was found that the modification leads to the change of the surface wettability depending on the time of modification. By use of a short time (tens of s) of modification the wettability can be reduced, use a longer time (hundreds of s) leads to increase of wettability. The surface morphology is significantly influenced by duration of modification, too. The plasma treatment involves the formation of the crystallites, whose number increases with increasing time of modification. On the basis of physicochemical properties evaluation, three types of the cells were cultivated on the selected samples. Cell-material interactions are strongly affected by material chemical structure and surface morphology. It was proved that the plasma treatment of PLLA has a positive effect on the adhesion, spreading, homogeneity of distribution and viability of all cultivated cells. This effect was even more apparent for the VSMCs and ASCs which homogeneously covered almost the whole surface of the substrate after 7 days of cultivation. The viability of these cells was high. (more than 98% for VSMCs, 89-96% for ASCs).

Název v anglickém jazyce

Effect of polymer modification on the cytocompatibility of human and rat cells

Popis výsledku anglicky

Tissue engineering includes use of a combination of cells, engineering and materials methods for the formation of new viable tissue for a medical purpose. Scaffolds represent important components for tissue engineering. For some applications, the great advantage of several polymeric materials is their cytocompatibility and susceptibility to biodegradation. Poly(L-lactic acid) (PLLA) is a fully biodegradable polymer which further degrades into H2O and CO2. The crucial factor for successful application of tissue replacements is the interaction of a graft with its surrounding tissue. This interaction involves cell adhesion, adsorption of extracellular molecules and support of cell proliferation. In this experiment, the effect of the surface modification of biodegradable polymer (performed by plasma treatment) on the various cell types was studied. The surface parameters and changes of the physicochemical properties of modified PLLA substrates were studied by different methods. Surface wettability was determined by goniometry, surface morphology and roughness study was performed with atomic force microscopy and chemical composition was determined using photoelectron spectroscopy. The physicochemical properties were studied in relation to cytocompatibility of human osteoblast (MG 63 cells), rat vascular smooth muscle cells (VSMC) and human stem cells (ASC) of the adipose tissue in vitro. A fluorescence microscopy was chosen to study and compare cell-material interaction. Important parameters of the cytocompatibility like adhesion, proliferation, viability, shape, spreading of the above-mentioned cells were evaluated. It was found that the modification leads to the change of the surface wettability depending on the time of modification. By use of a short time (tens of s) of modification the wettability can be reduced, use a longer time (hundreds of s) leads to increase of wettability. The surface morphology is significantly influenced by duration of modification, too. The plasma treatment involves the formation of the crystallites, whose number increases with increasing time of modification. On the basis of physicochemical properties evaluation, three types of the cells were cultivated on the selected samples. Cell-material interactions are strongly affected by material chemical structure and surface morphology. It was proved that the plasma treatment of PLLA has a positive effect on the adhesion, spreading, homogeneity of distribution and viability of all cultivated cells. This effect was even more apparent for the VSMCs and ASCs which homogeneously covered almost the whole surface of the substrate after 7 days of cultivation. The viability of these cells was high. (more than 98% for VSMCs, 89-96% for ASCs).

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20501 - Materials engineering

Projekt

<a href="/cs/project/GBP108%2F12%2FG108" target="_blank" >GBP108/12/G108: Příprava, modifikace a charakterizace materiálů zářením</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

International Journal of Materials and Metallurgical Engineering

ISBN

—

ISSN

2010-3778

e-ISSN

neuvedeno

Počet stran výsledku

6

Strana od-do

120-125

Název nakladatele

World Academy of Science, Engineering and Technology (WASET)

Místo vydání

Connecticut

Místo konání akce

Sydney

Datum konání akce

26. 1. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—