Black phosphorus nanoparticles as a novel fluorescent sensing platform for nucleic acid detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F17%3A43913407" target="_blank" >RIV/60461373:22310/17:43913407 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.rsc.org/en/content/articlehtml/2017/qm/c6qm00341a" target="_blank" >http://pubs.rsc.org/en/content/articlehtml/2017/qm/c6qm00341a</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c6qm00341a" target="_blank" >10.1039/c6qm00341a</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Black phosphorus nanoparticles as a novel fluorescent sensing platform for nucleic acid detection

Popis výsledku v původním jazyce

Two-dimensional black phosphorus is an emerging material. For the past two years, black phosphorus has attracted the attention of the scientific community due to its promising applications in electronic devices and biosensors. In this work, black phosphorus nanoparticles (BPNPs) derived from a facile, one-step synthesis method were successfully employed as nanofluorophores in the development of a fluorescent sensing platform for DNA detection, for the first time. This detection strategy exploits the differences in affinities of labelled single-stranded and double-stranded DNA oligonucleotides towards spontaneous self-assembly with black phosphorus nanoparticles. A wide linear detection range with good linearity (r = 0.91) spanning from 4 pM to 4000 pM was achieved with the constructed nanosensor, including the ability to distinguish triple nucleotide polymorphism. Moreover, high sensitivity was also demonstrated with a low limit of detection (LOD = 5.9 pM) and limit of quantification (LOQ = 19.7 pM). This paper demonstrates a proof of concept that black phosphorus possesses promising potential for biosensing applications, in particular as DNA nanobiosensors, and the reported findings would be significant in encouraging further research work into the development of biomedical devices and technologies based on black phosphorus.

Název v anglickém jazyce

Black phosphorus nanoparticles as a novel fluorescent sensing platform for nucleic acid detection

Popis výsledku anglicky

Two-dimensional black phosphorus is an emerging material. For the past two years, black phosphorus has attracted the attention of the scientific community due to its promising applications in electronic devices and biosensors. In this work, black phosphorus nanoparticles (BPNPs) derived from a facile, one-step synthesis method were successfully employed as nanofluorophores in the development of a fluorescent sensing platform for DNA detection, for the first time. This detection strategy exploits the differences in affinities of labelled single-stranded and double-stranded DNA oligonucleotides towards spontaneous self-assembly with black phosphorus nanoparticles. A wide linear detection range with good linearity (r = 0.91) spanning from 4 pM to 4000 pM was achieved with the constructed nanosensor, including the ability to distinguish triple nucleotide polymorphism. Moreover, high sensitivity was also demonstrated with a low limit of detection (LOD = 5.9 pM) and limit of quantification (LOQ = 19.7 pM). This paper demonstrates a proof of concept that black phosphorus possesses promising potential for biosensing applications, in particular as DNA nanobiosensors, and the reported findings would be significant in encouraging further research work into the development of biomedical devices and technologies based on black phosphorus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10402 - Inorganic and nuclear chemistry

Projekt

<a href="/cs/project/GA15-09001S" target="_blank" >GA15-09001S: Chemické modifikace materiálů na bázi grafenu: Syntéza grafanu a halogengrafenu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Chemistry Frontiers

ISSN

2052-1537

e-ISSN

—

Svazek periodika

1

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

1130-1136

Kód UT WoS článku

000412450600013

EID výsledku v databázi Scopus

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