Proteinase- sculptured 3D-printed graphene/polylactic acid electrodes as potential biosensing platforms: towards enzymatic modeling of 3D-printed structures dagger

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918021" target="_blank" >RIV/60461373:22310/19:43918021 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/19:PU133207

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlepdf/2019/nr/c9nr02754h" target="_blank" >https://pubs.rsc.org/en/content/articlepdf/2019/nr/c9nr02754h</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c9nr02754h" target="_blank" >10.1039/c9nr02754h</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteinase- sculptured 3D-printed graphene/polylactic acid electrodes as potential biosensing platforms: towards enzymatic modeling of 3D-printed structures dagger

Popis výsledku v původním jazyce

3D printing technologies are currently appealing for the research community due to their demonstrated versatility for different scientific applications. One of the most commonly used materials for 3D printing is polylactic acid (PLA), a biodegradable polymer that can be fully or partially digested by enzymes such as proteinase K. This work seeks to exploit PLA&apos;s biodegradability to selectively and reproducibly sculpt 3D-printed graphene/PLA surfaces to turn them into sensitive electroactive platforms. Proteinase K-catalyzed digestion of 3D-printed graphene/PLA electrodes is proposed as an environmentally friendly, highly controllable, and reproducible activation procedure of 3D-printed electrodes. Proteinase K digests PLA in a controllable fashion, exposing electroactive graphene sheets embedded within the 3D-printed structures to the solution and therefore achieving a tailorable electrode performance. A proof-of-concept biosensing application is proposed, based on the immobilization of enzyme alkaline phosphatase at the sculptured electrodes with the subsequent electrochemical detection of 1-naphthol in aqueous media. This work attempts to continue demonstrating the potential of 3D printing in electroanalytical applications, as well as to explore the exciting possibilities arising from merging biotechnological processes with these manufacturing procedures.

Název v anglickém jazyce

Proteinase- sculptured 3D-printed graphene/polylactic acid electrodes as potential biosensing platforms: towards enzymatic modeling of 3D-printed structures dagger

Popis výsledku anglicky

3D printing technologies are currently appealing for the research community due to their demonstrated versatility for different scientific applications. One of the most commonly used materials for 3D printing is polylactic acid (PLA), a biodegradable polymer that can be fully or partially digested by enzymes such as proteinase K. This work seeks to exploit PLA&apos;s biodegradability to selectively and reproducibly sculpt 3D-printed graphene/PLA surfaces to turn them into sensitive electroactive platforms. Proteinase K-catalyzed digestion of 3D-printed graphene/PLA electrodes is proposed as an environmentally friendly, highly controllable, and reproducible activation procedure of 3D-printed electrodes. Proteinase K digests PLA in a controllable fashion, exposing electroactive graphene sheets embedded within the 3D-printed structures to the solution and therefore achieving a tailorable electrode performance. A proof-of-concept biosensing application is proposed, based on the immobilization of enzyme alkaline phosphatase at the sculptured electrodes with the subsequent electrochemical detection of 1-naphthol in aqueous media. This work attempts to continue demonstrating the potential of 3D printing in electroanalytical applications, as well as to explore the exciting possibilities arising from merging biotechnological processes with these manufacturing procedures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/GA17-05421S" target="_blank" >GA17-05421S: Nové účinné membrány pro efektivní separace H2 / CO2 (HySME)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nanoscale

ISSN

2040-3364

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

25

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

12124-12131

Kód UT WoS článku

000475468100007

EID výsledku v databázi Scopus

2-s2.0-85068212735