Biocompatibility of Ar plasma-treated fluorinated ethylene propylene: Adhesion and viability of human keratinocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918503" target="_blank" >RIV/60461373:22310/19:43918503 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/19:43918503

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0928493118332521" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0928493118332521</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2019.02.100" target="_blank" >10.1016/j.msec.2019.02.100</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biocompatibility of Ar plasma-treated fluorinated ethylene propylene: Adhesion and viability of human keratinocytes

Popis výsledku v původním jazyce

Suitable surface modification of synthetic polymers is vital for improvement of cell adhesion to such materials. To that effect, reproducible modification methods such as argon plasma treatment are advantageous. We have previously described a significant improvement of cell adhesion and proliferation on Ar-plasma modified fluorinated ethylene propylene (FEP). In the experiments presented here, we sought to gain a closer insight into cell adhesion on modified FEP matrices by means of analysis of focal adhesions (FM) as markers of adhesion. The length of FM visualized by immunofluorescent labelling of talin 1 in human keratinocytes (HaCaT) cultured on selected Ar plasma modified matrices was greater at 24 h than at 72 h. The number of FM per cell with FM increased for some of the modified surfaces during the 72 h period and the percentage of cells with FM mildly decreased. Furthermore, we analyzed the percentage of live HaCaT cells on the tested matrices by means of live/dead staining, with cells reaching almost 100% viability on modified FEP matrices. Altogether, Ar plasma modification positively affected viability and adhesion of HaCaT cells on FEP. Both these features may be related because formation of focal adhesions not only enables cell adhesion but also affects intracellular signaling. We also show that the assessment of suitable surface modification of biocompatible materials should be more complex than mere evaluation of viability or number of the cells on the surface.

Název v anglickém jazyce

Biocompatibility of Ar plasma-treated fluorinated ethylene propylene: Adhesion and viability of human keratinocytes

Popis výsledku anglicky

Suitable surface modification of synthetic polymers is vital for improvement of cell adhesion to such materials. To that effect, reproducible modification methods such as argon plasma treatment are advantageous. We have previously described a significant improvement of cell adhesion and proliferation on Ar-plasma modified fluorinated ethylene propylene (FEP). In the experiments presented here, we sought to gain a closer insight into cell adhesion on modified FEP matrices by means of analysis of focal adhesions (FM) as markers of adhesion. The length of FM visualized by immunofluorescent labelling of talin 1 in human keratinocytes (HaCaT) cultured on selected Ar plasma modified matrices was greater at 24 h than at 72 h. The number of FM per cell with FM increased for some of the modified surfaces during the 72 h period and the percentage of cells with FM mildly decreased. Furthermore, we analyzed the percentage of live HaCaT cells on the tested matrices by means of live/dead staining, with cells reaching almost 100% viability on modified FEP matrices. Altogether, Ar plasma modification positively affected viability and adhesion of HaCaT cells on FEP. Both these features may be related because formation of focal adhesions not only enables cell adhesion but also affects intracellular signaling. We also show that the assessment of suitable surface modification of biocompatible materials should be more complex than mere evaluation of viability or number of the cells on the surface.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20501 - Materials engineering

Projekt

<a href="/cs/project/GBP108%2F12%2FG108" target="_blank" >GBP108/12/G108: Příprava, modifikace a charakterizace materiálů zářením</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials science and engineering C

ISSN

0928-4931

e-ISSN

—

Svazek periodika

100

Číslo periodika v rámci svazku

JUL 2019

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

269-275

Kód UT WoS článku

000466059700026

EID výsledku v databázi Scopus

2-s2.0-85062466390