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Preparation and Investigation of Silver Nanoparticle-Antibody Bioconjugates for Electrochemicals Immunoassay of Tick-Borne Encephalitis

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918679" target="_blank" >RIV/60461373:22310/19:43918679 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://www.mdpi.com/1424-8220/19/9/2103" target="_blank" >https://www.mdpi.com/1424-8220/19/9/2103</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3390/s19092103" target="_blank" >10.3390/s19092103</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Preparation and Investigation of Silver Nanoparticle-Antibody Bioconjugates for Electrochemicals Immunoassay of Tick-Borne Encephalitis

  • Popis výsledku v původním jazyce

    A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (Ab@AgNP). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold–carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their Ab@AgNP. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for Ab@AgNP preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL−1 to 1600 IU·mL−1, with a detection limit of 50 IU·mL−1 . The coefficient of determination (r2) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.

  • Název v anglickém jazyce

    Preparation and Investigation of Silver Nanoparticle-Antibody Bioconjugates for Electrochemicals Immunoassay of Tick-Borne Encephalitis

  • Popis výsledku anglicky

    A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (Ab@AgNP). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold–carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their Ab@AgNP. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for Ab@AgNP preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL−1 to 1600 IU·mL−1, with a detection limit of 50 IU·mL−1 . The coefficient of determination (r2) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2019

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Sensors

  • ISSN

    1424-8220

  • e-ISSN

  • Svazek periodika

    19

  • Číslo periodika v rámci svazku

    9

  • Stát vydavatele periodika

    CH - Švýcarská konfederace

  • Počet stran výsledku

    10

  • Strana od-do

  • Kód UT WoS článku

    000469766800147

  • EID výsledku v databázi Scopus

    2-s2.0-85065758889