Electrochemical immunoassay for the detection of antibodies to tick-borne encephalitis virus by using various types of bioconjugates based on silver nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F20%3A43920986" target="_blank" >RIV/60461373:22310/20:43920986 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/20:10418225

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S1567539420300256?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1567539420300256?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioelechem.2020.107576" target="_blank" >10.1016/j.bioelechem.2020.107576</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical immunoassay for the detection of antibodies to tick-borne encephalitis virus by using various types of bioconjugates based on silver nanoparticles

Popis výsledku v původním jazyce

This work reports for the first time a significantly improved and simplified electrochemical immunoassay to detect antibodies to tick-borne encephalitis virus (TBEV) using a 96-well microtiter plate as a platform for immobilization and silver nanoparticles (AgNPs) as electrochemical labels. The electrochemical assay is performed by detecting the elemental silver oxidation signal where the electroactive signalling silver species are released from the bioconjugates (Ab@AgNP, AbS@AgNP, and ProteinA@AgNP). For this purpose, AgNPs were synthesized and further tagged with biomolecules (antibodies to TBEV, cleaved antibodies to TBEV, and protein A). Signal is read by linear sweep anodic stripping voltammetry (LSASV) of silver ions (through the electrochemical stripping of accumulated elemental silver) on a graphite electrode (GE). AbS@AgNP was chosen as the best option for the new electrochemical immunoassay. The results of electrochemical measurements demonstrated that voltammetric signal increased with the increasing concentration of target antibodies to TBEV within the range from 100 to 1600 IU mL(-1), with a detection limit of 90 IU mL(-1). To verify the practical application of the novel electrochemical immunosensor, the quantity of immunoglobulins against TBEV in human serum was checked. The results may contribute to the development of alternative methods for monitoring TBEV in biological fluids. (C) 2020 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Electrochemical immunoassay for the detection of antibodies to tick-borne encephalitis virus by using various types of bioconjugates based on silver nanoparticles

Popis výsledku anglicky

This work reports for the first time a significantly improved and simplified electrochemical immunoassay to detect antibodies to tick-borne encephalitis virus (TBEV) using a 96-well microtiter plate as a platform for immobilization and silver nanoparticles (AgNPs) as electrochemical labels. The electrochemical assay is performed by detecting the elemental silver oxidation signal where the electroactive signalling silver species are released from the bioconjugates (Ab@AgNP, AbS@AgNP, and ProteinA@AgNP). For this purpose, AgNPs were synthesized and further tagged with biomolecules (antibodies to TBEV, cleaved antibodies to TBEV, and protein A). Signal is read by linear sweep anodic stripping voltammetry (LSASV) of silver ions (through the electrochemical stripping of accumulated elemental silver) on a graphite electrode (GE). AbS@AgNP was chosen as the best option for the new electrochemical immunoassay. The results of electrochemical measurements demonstrated that voltammetric signal increased with the increasing concentration of target antibodies to TBEV within the range from 100 to 1600 IU mL(-1), with a detection limit of 90 IU mL(-1). To verify the practical application of the novel electrochemical immunosensor, the quantity of immunoglobulins against TBEV in human serum was checked. The results may contribute to the development of alternative methods for monitoring TBEV in biological fluids. (C) 2020 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GC20-01417J" target="_blank" >GC20-01417J: Nové přístupy zabraňující pasivaci elektrod při monitorování environmentálních organických polutantů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioelektrochemistry

ISSN

1567-5394

e-ISSN

—

Svazek periodika

135

Číslo periodika v rámci svazku

october

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000579730600015

EID výsledku v databázi Scopus

2-s2.0-85085658511