Structurally controlled cell growth on modified polymer surfaces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43919415" target="_blank" >RIV/60461373:22310/19:43919415 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/19:43919415

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0042207X18320694?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0042207X18320694?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vacuum.2019.108956" target="_blank" >10.1016/j.vacuum.2019.108956</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structurally controlled cell growth on modified polymer surfaces

Popis výsledku v původním jazyce

Cells are subject to various stimuli in their natural environment, which substrates for their culturing should be aspiring to imitate, because it is necessary for their correct function. Substrate topography is one of the stimuli as the cells in vivo are directed to only grow according to the extracellular matrix. In this work, we explored methods of microstructured modification of polymer substrates using masked cathode sputtering of Au and plasma etching to produce materials with contrasting cytocompatibility surface microstructure. Au was sputtered on poly-L-lactic acid through a metal mask. The microstructure was successfully prepared on the sample surface, but growth of the mouse fibroblast cells (L929) used for the cytocompatibility study did not respect features of the prepared material. Polytetrafluoroethylene was exposed to plasma treatment, which leads to the ablation of the surface polymer layer causing changes of its surface morphology and chemistry and improves its cytocompatibility. The cell growth was following the prepared microstructure in this case, but the experiment was found to be strongly dependent on the parameters of the cell cultivation, because with longer cultivation times the prepared structure was covered with a great amount of the growing cells.

Název v anglickém jazyce

Structurally controlled cell growth on modified polymer surfaces

Popis výsledku anglicky

Cells are subject to various stimuli in their natural environment, which substrates for their culturing should be aspiring to imitate, because it is necessary for their correct function. Substrate topography is one of the stimuli as the cells in vivo are directed to only grow according to the extracellular matrix. In this work, we explored methods of microstructured modification of polymer substrates using masked cathode sputtering of Au and plasma etching to produce materials with contrasting cytocompatibility surface microstructure. Au was sputtered on poly-L-lactic acid through a metal mask. The microstructure was successfully prepared on the sample surface, but growth of the mouse fibroblast cells (L929) used for the cytocompatibility study did not respect features of the prepared material. Polytetrafluoroethylene was exposed to plasma treatment, which leads to the ablation of the surface polymer layer causing changes of its surface morphology and chemistry and improves its cytocompatibility. The cell growth was following the prepared microstructure in this case, but the experiment was found to be strongly dependent on the parameters of the cell cultivation, because with longer cultivation times the prepared structure was covered with a great amount of the growing cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20501 - Materials engineering

Projekt

<a href="/cs/project/GA17-00939S" target="_blank" >GA17-00939S: Příprava a charakterizace tenkých vrstev a nanostruktur se specifickými magnetickými vlastnostmi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

VACUUM

ISSN

0042-207X

e-ISSN

—

Svazek periodika

169

Číslo periodika v rámci svazku

NOV 2019

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

"108956/1"-7

Kód UT WoS článku

000494887000001

EID výsledku v databázi Scopus

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