Hyaluronic Acid Fibres in Solid Phase Peptide Synthesis - Their Properties, Morphology and Stability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F20%3A43920576" target="_blank" >RIV/60461373:22310/20:43920576 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/20:43920576

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s12221-020-0075-6" target="_blank" >https://link.springer.com/article/10.1007/s12221-020-0075-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12221-020-0075-6" target="_blank" >10.1007/s12221-020-0075-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hyaluronic Acid Fibres in Solid Phase Peptide Synthesis - Their Properties, Morphology and Stability

Popis výsledku v původním jazyce

Different methods and materials can be used for peptide synthesis. One of the most common approaches is synthesis on a solid phase. In this paper, we describe fibres based on hyaluronic acid (HA) and their properties under solid-phase peptide synthesis (SPPS) conditions. The fibres were prepared by wet spinning technology and were used to study their stability and mechanical properties under the reaction conditions for direct peptide synthesis or conjugation with a pre-synthesized peptide. Fibres in the form of a non-woven textile were then used as a proof of concept for the adhesion of cells on short cell-adhesion peptides. The methods, solvents and reaction conditions used showed no significant influence on the necessary stability of the HA fibres when the Fmoc/tBu strategy was applied. Furthermore, the stability of the HA fibres in aqueous solution was strongly improved. The stabilization and presence of cell-adhesive peptides supported the adhesion of cardiomyocyte cells, which confirmed the functionality of the modified textile. Therefore, HA carriers bearing peptides could be advantageously employed in various biomedical applications as repair patches.

Název v anglickém jazyce

Hyaluronic Acid Fibres in Solid Phase Peptide Synthesis - Their Properties, Morphology and Stability

Popis výsledku anglicky

Different methods and materials can be used for peptide synthesis. One of the most common approaches is synthesis on a solid phase. In this paper, we describe fibres based on hyaluronic acid (HA) and their properties under solid-phase peptide synthesis (SPPS) conditions. The fibres were prepared by wet spinning technology and were used to study their stability and mechanical properties under the reaction conditions for direct peptide synthesis or conjugation with a pre-synthesized peptide. Fibres in the form of a non-woven textile were then used as a proof of concept for the adhesion of cells on short cell-adhesion peptides. The methods, solvents and reaction conditions used showed no significant influence on the necessary stability of the HA fibres when the Fmoc/tBu strategy was applied. Furthermore, the stability of the HA fibres in aqueous solution was strongly improved. The stabilization and presence of cell-adhesive peptides supported the adhesion of cardiomyocyte cells, which confirmed the functionality of the modified textile. Therefore, HA carriers bearing peptides could be advantageously employed in various biomedical applications as repair patches.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fibers and Polymers

ISSN

1229-9197

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

KR - Korejská republika

Počet stran výsledku

11

Strana od-do

2707-2717

Kód UT WoS článku

000602558200001

EID výsledku v databázi Scopus

2-s2.0-85098096473