Carboxyethyl-functionalized 3D porous polypyrrole synthesized using a porogen-free method for covalent immobilization of urease
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F20%3A43921851" target="_blank" >RIV/60461373:22310/20:43921851 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60461373:22330/20:43921851 RIV/60461373:22340/20:43921851 RIV/60461373:22310/21:43922845 RIV/60461373:22330/21:43922845 RIV/60461373:22340/21:43922845
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S1387181120306909" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1387181120306909</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Carboxyethyl-functionalized 3D porous polypyrrole synthesized using a porogen-free method for covalent immobilization of urease
Popis výsledku v původním jazyce
The immobilization of enzymes onto porous supports is a common strategy for obtaining improved stability, fast product separation, enzyme reusability, and, ultimately, lower operating costs. Therefore, the development of new supports with specific surface functionalities that enable the covalent attachment of enzymes is of significant interest. Herein, stable three-dimensional (3D) porous materials were synthesized from polypyrrole by a simple template approach and used as an immobilization support for urease. The template method entails the use of polypyrrole nanoparticle building blocks, onto which a carboxylic acid-functionalized pyrrole monomer was polymerized, forming a 3D porous structure with tunable pore size distribution. Scanning electron microscopy (SEM) images, together with static light scattering (SLS), revealed the 3D porous nature of the materials. The properties of both the supports and the immobilized enzyme were characterized using a combination of tech-niques. The ability of the bioconjugated urease to catalyze the hydrolysis of urea into carbon dioxide and ammonia was then tested. The immobilized enzyme exhibited good catalytic activity, stability and reusability. Overall, these results suggest that such 3D porous materials with chemically accessible surfaces have consider-able potential for use as biocatalyst supports.
Název v anglickém jazyce
Carboxyethyl-functionalized 3D porous polypyrrole synthesized using a porogen-free method for covalent immobilization of urease
Popis výsledku anglicky
The immobilization of enzymes onto porous supports is a common strategy for obtaining improved stability, fast product separation, enzyme reusability, and, ultimately, lower operating costs. Therefore, the development of new supports with specific surface functionalities that enable the covalent attachment of enzymes is of significant interest. Herein, stable three-dimensional (3D) porous materials were synthesized from polypyrrole by a simple template approach and used as an immobilization support for urease. The template method entails the use of polypyrrole nanoparticle building blocks, onto which a carboxylic acid-functionalized pyrrole monomer was polymerized, forming a 3D porous structure with tunable pore size distribution. Scanning electron microscopy (SEM) images, together with static light scattering (SLS), revealed the 3D porous nature of the materials. The properties of both the supports and the immobilized enzyme were characterized using a combination of tech-niques. The ability of the bioconjugated urease to catalyze the hydrolysis of urea into carbon dioxide and ammonia was then tested. The immobilized enzyme exhibited good catalytic activity, stability and reusability. Overall, these results suggest that such 3D porous materials with chemically accessible surfaces have consider-able potential for use as biocatalyst supports.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
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OECD FORD obor
10404 - Polymer science
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Microporous and mesoporous materials
ISSN
1387-1811
e-ISSN
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Svazek periodika
311
Číslo periodika v rámci svazku
October
Stát vydavatele periodika
BE - Belgické království
Počet stran výsledku
11
Strana od-do
1-11
Kód UT WoS článku
000600421100004
EID výsledku v databázi Scopus
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