Carboxyethyl-functionalized 3D porous polypyrrole synthesized using a porogen-free method for covalent immobilization of urease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F21%3A43922845" target="_blank" >RIV/60461373:22310/21:43922845 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22310/20:43921851 RIV/60461373:22330/20:43921851 RIV/60461373:22340/20:43921851 RIV/60461373:22330/21:43922845 RIV/60461373:22340/21:43922845

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1387181120306909?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1387181120306909?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.micromeso.2020.110690" target="_blank" >10.1016/j.micromeso.2020.110690</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carboxyethyl-functionalized 3D porous polypyrrole synthesized using a porogen-free method for covalent immobilization of urease

Popis výsledku v původním jazyce

The immobilization of enzymes onto porous supports is a common strategy for obtaining improved stability, fast product separation, enzyme reusability, and, ultimately, lower operating costs. Therefore, the development of new supports with specific surface functionalities that enable the covalent attachment of enzymes is of significant interest. Herein, stable three-dimensional (3D) porous materials were synthesized from polypyrrole by a simple template approach and used as an immobilization support for urease. The template method entails the use of polypyrrole nanoparticle building blocks, onto which a carboxylic acid-functionalized pyrrole monomer was polymerized, forming a 3D porous structure with tunable pore size distribution. Scanning electron microscopy (SEM) images, together with static light scattering (SLS), revealed the 3D porous nature of the materials. The properties of both the supports and the immobilized enzyme were characterized using a combination of techniques. The ability of the bioconjugated urease to catalyze the hydrolysis of urea into carbon dioxide and ammonia was then tested. The immobilized enzyme exhibited good catalytic activity, stability and reusability. Overall, these results suggest that such 3D porous materials with chemically accessible surfaces have considerable potential for use as biocatalyst supports.

Název v anglickém jazyce

Carboxyethyl-functionalized 3D porous polypyrrole synthesized using a porogen-free method for covalent immobilization of urease

Popis výsledku anglicky

The immobilization of enzymes onto porous supports is a common strategy for obtaining improved stability, fast product separation, enzyme reusability, and, ultimately, lower operating costs. Therefore, the development of new supports with specific surface functionalities that enable the covalent attachment of enzymes is of significant interest. Herein, stable three-dimensional (3D) porous materials were synthesized from polypyrrole by a simple template approach and used as an immobilization support for urease. The template method entails the use of polypyrrole nanoparticle building blocks, onto which a carboxylic acid-functionalized pyrrole monomer was polymerized, forming a 3D porous structure with tunable pore size distribution. Scanning electron microscopy (SEM) images, together with static light scattering (SLS), revealed the 3D porous nature of the materials. The properties of both the supports and the immobilized enzyme were characterized using a combination of techniques. The ability of the bioconjugated urease to catalyze the hydrolysis of urea into carbon dioxide and ammonia was then tested. The immobilized enzyme exhibited good catalytic activity, stability and reusability. Overall, these results suggest that such 3D porous materials with chemically accessible surfaces have considerable potential for use as biocatalyst supports.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20401 - Chemical engineering (plants, products)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microporous and mesoporous materials

ISSN

1387-1811

e-ISSN

—

Svazek periodika

311

Číslo periodika v rámci svazku

FEB 2021

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

110690

Kód UT WoS článku

000600421100004

EID výsledku v databázi Scopus

2-s2.0-85092784477