Genomic Damage Induced in Nicotiana tabacum L. Plants by Colloidal Solution with Silver and Gold Nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F21%3A43922187" target="_blank" >RIV/60461373:22310/21:43922187 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/21:00545280 RIV/60461373:22330/21:43922187 RIV/60461373:22340/21:43922187

Výsledek na webu

<a href="https://www.mdpi.com/2223-7747/10/6/1260/htm" target="_blank" >https://www.mdpi.com/2223-7747/10/6/1260/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/plants10061260" target="_blank" >10.3390/plants10061260</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genomic Damage Induced in Nicotiana tabacum L. Plants by Colloidal Solution with Silver and Gold Nanoparticles

Popis výsledku v původním jazyce

Tobacco seedlings (Nicotiana tabacum L cv. Wisconsin 38) were treated for 24 h with colloidal solution of silver and gold nanoparticles (AgNPs and AuNPs) of different size or cultivated for 8 weeks on soil polluted with these NPs. DNA damage in leaf and roots nuclei was evaluated by the comet assay. AgNPs of the size 22-25 nm at concentrations higher than 50 mg.L-1 significantly increased the tail moments (TM) values in leaf nuclei compared to the negative control. Ag nanoparticles of smaller size 12-15 nm caused a slight increase in tail moment without significant difference from the negative control. The opposite effect of AgNPs was observed on roots. The increasing tail moment was registered for smaller NPs. Similar results were observed for AuNPs at a concentration of 100 mg.L-1. DNA damaging effects after growing tobacco plants for 8 weeks in soil polluted with AgNPs and AuNPs of different size and concentrations were observed. While lower concentrations of both types of particles had no effect on the integrity of DNA, concentration of 30 mg.kg(-1) of AgNPs caused significant DNA damage in leaves of tobacco plants. AuNPs had no effect even at the highest concentration. The content of Ag was determined by ICP-MS in above-ground part of plants (leaves) after 8 weeks of growth in soil with 30 mg.kg(-1). AgNPs and was 2.720 +/- 0.408 mu g.g(-1). Long term effect is much less harmful probably due to the plant restoration capability.

Název v anglickém jazyce

Genomic Damage Induced in Nicotiana tabacum L. Plants by Colloidal Solution with Silver and Gold Nanoparticles

Popis výsledku anglicky

Tobacco seedlings (Nicotiana tabacum L cv. Wisconsin 38) were treated for 24 h with colloidal solution of silver and gold nanoparticles (AgNPs and AuNPs) of different size or cultivated for 8 weeks on soil polluted with these NPs. DNA damage in leaf and roots nuclei was evaluated by the comet assay. AgNPs of the size 22-25 nm at concentrations higher than 50 mg.L-1 significantly increased the tail moments (TM) values in leaf nuclei compared to the negative control. Ag nanoparticles of smaller size 12-15 nm caused a slight increase in tail moment without significant difference from the negative control. The opposite effect of AgNPs was observed on roots. The increasing tail moment was registered for smaller NPs. Similar results were observed for AuNPs at a concentration of 100 mg.L-1. DNA damaging effects after growing tobacco plants for 8 weeks in soil polluted with AgNPs and AuNPs of different size and concentrations were observed. While lower concentrations of both types of particles had no effect on the integrity of DNA, concentration of 30 mg.kg(-1) of AgNPs caused significant DNA damage in leaves of tobacco plants. AuNPs had no effect even at the highest concentration. The content of Ag was determined by ICP-MS in above-ground part of plants (leaves) after 8 weeks of growth in soil with 30 mg.kg(-1). AgNPs and was 2.720 +/- 0.408 mu g.g(-1). Long term effect is much less harmful probably due to the plant restoration capability.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/GA17-10907S" target="_blank" >GA17-10907S: Dopad nanočástic ušlechtilých kovů na životní prostředí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plants

ISSN

2223-7747

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

000666150800001

EID výsledku v databázi Scopus

2-s2.0-85108142698