Effect of buffer in simulated body fluid on morphology and crystallinity of hydroxyapatite precipitated on 45S5 bioactive glass-derived glass-ceramic scaffolds: comparison of Good´s buffer system and tris

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F21%3A43922237" target="_blank" >RIV/60461373:22310/21:43922237 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2468519421001075" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2468519421001075</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mtchem.2021.100527" target="_blank" >10.1016/j.mtchem.2021.100527</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of buffer in simulated body fluid on morphology and crystallinity of hydroxyapatite precipitated on 45S5 bioactive glass-derived glass-ceramic scaffolds: comparison of Good´s buffer system and tris

Popis výsledku v původním jazyce

In vitro tests that verify the ability of a material to form bone-like apatite (HAp) use a solution that imitates the inorganic part of blood plasma, simulated body fluid (SBF). During such tests, it is necessary to maintain a neutral pH, for which purpose the International Standards Organization recommends the TRIS buffer (ISO - 23317:2014). To do this, TRIS buffer must remain inert, but, as we have previously reported, TRIS interacts with highly bioreactive materials (such as 45S5 Bioglass derived scaffolds), thereby accelerating scaffold dissolution. In the search for an alternative to TRIS, we have also recently published results for the HEPES and MOPS buffers, which were also unable to maintain a neutral pH in SBF. Thus, we here continue our search for a more suitable Good´s buffer by comparing BES (N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) and TES (2-[(2-Hydroxy-1,1-bis(hydroxymethyl) ethyl) amino] ethanesulfonic acid) with TRIS. 45S5-derived glass-ceramic scaffolds were tested under static-dynamic conditions for bone-like apatite formation using SBF+BES and SBF+TES solutions. The pH measurements, leachate analysis [AAS (Ca2+, SiIV), UV-VIS spectrophotometry (PO4)3-] and scaffold analysis (XRD, XRF, SEM/EDS, BET) all showed that both BES and TES quickly interacted with the tested material. Moreover, the kinetics of the glass-ceramic dissolution affected the crystallinity and morphology of the precipitated HAp. Based on our previous results and the present data, it appears that Good´s buffers are not suitable for the in vitro testing of bioactivity of highly bioreactive materials.

Název v anglickém jazyce

Effect of buffer in simulated body fluid on morphology and crystallinity of hydroxyapatite precipitated on 45S5 bioactive glass-derived glass-ceramic scaffolds: comparison of Good´s buffer system and tris

Popis výsledku anglicky

In vitro tests that verify the ability of a material to form bone-like apatite (HAp) use a solution that imitates the inorganic part of blood plasma, simulated body fluid (SBF). During such tests, it is necessary to maintain a neutral pH, for which purpose the International Standards Organization recommends the TRIS buffer (ISO - 23317:2014). To do this, TRIS buffer must remain inert, but, as we have previously reported, TRIS interacts with highly bioreactive materials (such as 45S5 Bioglass derived scaffolds), thereby accelerating scaffold dissolution. In the search for an alternative to TRIS, we have also recently published results for the HEPES and MOPS buffers, which were also unable to maintain a neutral pH in SBF. Thus, we here continue our search for a more suitable Good´s buffer by comparing BES (N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) and TES (2-[(2-Hydroxy-1,1-bis(hydroxymethyl) ethyl) amino] ethanesulfonic acid) with TRIS. 45S5-derived glass-ceramic scaffolds were tested under static-dynamic conditions for bone-like apatite formation using SBF+BES and SBF+TES solutions. The pH measurements, leachate analysis [AAS (Ca2+, SiIV), UV-VIS spectrophotometry (PO4)3-] and scaffold analysis (XRD, XRF, SEM/EDS, BET) all showed that both BES and TES quickly interacted with the tested material. Moreover, the kinetics of the glass-ceramic dissolution affected the crystallinity and morphology of the precipitated HAp. Based on our previous results and the present data, it appears that Good´s buffers are not suitable for the in vitro testing of bioactivity of highly bioreactive materials.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20504 - Ceramics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Today Chemistry

ISSN

2468-5194

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

1-9

Kód UT WoS článku

000696937300014

EID výsledku v databázi Scopus

2-s2.0-85111318825