Interaction of MOPS buffer with glass-ceramic scaffold: Effect of (PO4)(3-) ions in SBF on kinetics and morphology of formatted hydroxyapatite

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388980%3A_____%2F20%3A00518626" target="_blank" >RIV/61388980:_____/20:00518626 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22310/19:43917990

Výsledek na webu

<a href="http://hdl.handle.net/11104/0308792" target="_blank" >http://hdl.handle.net/11104/0308792</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jbm.b.34530" target="_blank" >10.1002/jbm.b.34530</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of MOPS buffer with glass-ceramic scaffold: Effect of (PO4)(3-) ions in SBF on kinetics and morphology of formatted hydroxyapatite

Popis výsledku v původním jazyce

The international standard ISO 23317:2014 for the in vitro testing of inorganic biomaterials in simulated body fluid (SBF) uses TRIS buffer to maintain neutral pH. In our previous papers, we investigated the interaction of a glass-ceramic scaffold with TRIS and HEPES buffers. Both of them speeded up glass-ceramic dissolution and hydroxyapatite (HAp) precipitation, thereby demonstrating their unsuitability for the in vitro testing of highly reactive biomaterials. In this article, we tested MOPS buffer (3-[N-morpholino] propanesulfonic acid), another amino acid from the group of “Goods buffers“. A highly reactive glass-ceramic scaffold (derived from Bioglass (R)) was exposed to SBF under static-dynamic conditions for 13/15 days. The kinetics and morphology of the newly precipitated HAp were studied using two different concentrations of (PO4)(3-) ions in SBF. The pH value and the Si-IV, Ca2+, and (PO4)(3-) concentrations in the SBF leachate samples were measured every day (AAS, spectrophotometry). The glass-ceramic scaffold was monitored by SEM/EDS, XRD, WD-XRF, and BET before and after 1, 3, 7, 11, and 13/15 days of exposure. As in the case of TRIS and HEPES, the preferential dissolution of the glass-ceramic crystalline phase (Combeite) was observed, but less intensively. The lower concentration of (PO4)(3-) ions slowed down the kinetics of HAp precipitation, thereby causing the disintegration of the scaffold structure. This phenomenon shows that the HAp phase was predominately generated by the presence of (PO4)(3-) ions in the SBF, not in the glass-ceramic material. Irrespective of this, MOPS buffer is not suitable for the maintenance of pH in SBF.

Název v anglickém jazyce

Interaction of MOPS buffer with glass-ceramic scaffold: Effect of (PO4)(3-) ions in SBF on kinetics and morphology of formatted hydroxyapatite

Popis výsledku anglicky

The international standard ISO 23317:2014 for the in vitro testing of inorganic biomaterials in simulated body fluid (SBF) uses TRIS buffer to maintain neutral pH. In our previous papers, we investigated the interaction of a glass-ceramic scaffold with TRIS and HEPES buffers. Both of them speeded up glass-ceramic dissolution and hydroxyapatite (HAp) precipitation, thereby demonstrating their unsuitability for the in vitro testing of highly reactive biomaterials. In this article, we tested MOPS buffer (3-[N-morpholino] propanesulfonic acid), another amino acid from the group of “Goods buffers“. A highly reactive glass-ceramic scaffold (derived from Bioglass (R)) was exposed to SBF under static-dynamic conditions for 13/15 days. The kinetics and morphology of the newly precipitated HAp were studied using two different concentrations of (PO4)(3-) ions in SBF. The pH value and the Si-IV, Ca2+, and (PO4)(3-) concentrations in the SBF leachate samples were measured every day (AAS, spectrophotometry). The glass-ceramic scaffold was monitored by SEM/EDS, XRD, WD-XRF, and BET before and after 1, 3, 7, 11, and 13/15 days of exposure. As in the case of TRIS and HEPES, the preferential dissolution of the glass-ceramic crystalline phase (Combeite) was observed, but less intensively. The lower concentration of (PO4)(3-) ions slowed down the kinetics of HAp precipitation, thereby causing the disintegration of the scaffold structure. This phenomenon shows that the HAp phase was predominately generated by the presence of (PO4)(3-) ions in the SBF, not in the glass-ceramic material. Irrespective of this, MOPS buffer is not suitable for the maintenance of pH in SBF.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10402 - Inorganic and nuclear chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biomedical Materials Research. Part B

ISSN

1552-4973

e-ISSN

—

Svazek periodika

108

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1888-1896

Kód UT WoS článku

000502699200001

EID výsledku v databázi Scopus

2-s2.0-85076742241