Surface modification of carbon dots with tetraalkylammonium moieties for fine tuning their antibacterial activity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F22%3A43925990" target="_blank" >RIV/60461373:22310/22:43925990 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.msec.2022.112697" target="_blank" >https://doi.org/10.1016/j.msec.2022.112697</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2022.112697" target="_blank" >10.1016/j.msec.2022.112697</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Surface modification of carbon dots with tetraalkylammonium moieties for fine tuning their antibacterial activity

Popis výsledku v původním jazyce

The widespread of bacterial infections including biofilms drives the never-ending quest for new antimicrobial agents. Among the great variety of nanomaterials, carbon dots (CDs) are the most promising antibacterial material, but still require the adjustment of their surface properties for enhanced activity. In this contribution, we report a facile functionalization method of carbon dots (CDs) by tetraalkylammonium moieties using diazonium chemistry to improve their antibacterial activity against Gram-positive and Gram-negative bacteria. CDs were modified by novel diazonium salts bearing tetraalkylammonium moieties (TAA) with different alkyl chains (C2, C4, C9, C12) for the optimization of antibacterial activity. Variation of the alkyl chain allows to reach the significant antibacterial effect for CDs-C9 towards Gram-positive Staphylococcus aureus (S. aureus) (MIC = 3.09 ± 1.10 μg mL−1) and Gram-negative Escherichia coli (E. coli) (MIC = 7.93 ± 0.17 μg mL−1) bacteria. The antibacterial mechanism of CDs-C9 is ascribed to the balance between the positive charge and hydrophobicity of the alkyl chains. TAA moieties are responsible for enhanced adherence on the bacterial cell membrane, its penetration and disturbance of physiological metabolism. CDs-C9 were not effective in the generation of reactive oxygen species excluding the oxidative damage mechanism. In addition, CDs-C9 effectively promoted the antibiofilm treatment of S. aureus and E. coli biofilms outperforming previously-reported CDs in terms of treatment duration and minimal inhibitory concentration. The good biocompatibility of CDs-C9 was demonstrated on mouse fibroblast (NIH/3T3), HeLa and U-87 MG cell lines for concentrations up to 256 μg mL−1. Collectively, our work highlights the correlation between the surface chemistry of CDs and their antimicrobial performance. © 2022 Elsevier B.V.

Název v anglickém jazyce

Surface modification of carbon dots with tetraalkylammonium moieties for fine tuning their antibacterial activity

Popis výsledku anglicky

The widespread of bacterial infections including biofilms drives the never-ending quest for new antimicrobial agents. Among the great variety of nanomaterials, carbon dots (CDs) are the most promising antibacterial material, but still require the adjustment of their surface properties for enhanced activity. In this contribution, we report a facile functionalization method of carbon dots (CDs) by tetraalkylammonium moieties using diazonium chemistry to improve their antibacterial activity against Gram-positive and Gram-negative bacteria. CDs were modified by novel diazonium salts bearing tetraalkylammonium moieties (TAA) with different alkyl chains (C2, C4, C9, C12) for the optimization of antibacterial activity. Variation of the alkyl chain allows to reach the significant antibacterial effect for CDs-C9 towards Gram-positive Staphylococcus aureus (S. aureus) (MIC = 3.09 ± 1.10 μg mL−1) and Gram-negative Escherichia coli (E. coli) (MIC = 7.93 ± 0.17 μg mL−1) bacteria. The antibacterial mechanism of CDs-C9 is ascribed to the balance between the positive charge and hydrophobicity of the alkyl chains. TAA moieties are responsible for enhanced adherence on the bacterial cell membrane, its penetration and disturbance of physiological metabolism. CDs-C9 were not effective in the generation of reactive oxygen species excluding the oxidative damage mechanism. In addition, CDs-C9 effectively promoted the antibiofilm treatment of S. aureus and E. coli biofilms outperforming previously-reported CDs in terms of treatment duration and minimal inhibitory concentration. The good biocompatibility of CDs-C9 was demonstrated on mouse fibroblast (NIH/3T3), HeLa and U-87 MG cell lines for concentrations up to 256 μg mL−1. Collectively, our work highlights the correlation between the surface chemistry of CDs and their antimicrobial performance. © 2022 Elsevier B.V.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20501 - Materials engineering

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomaterials Advances

ISSN

2772-9508

e-ISSN

2772-9508

Svazek periodika

134

Číslo periodika v rámci svazku

MAR 2022

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

"112697/1"-12

Kód UT WoS článku

000811744700015

EID výsledku v databázi Scopus

2-s2.0-85130374463