Impedimetric detection of gut-derived metabolites using 2D Germanene-based materials

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F24%3A43927575" target="_blank" >RIV/60461373:22310/24:43927575 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0039914023012602" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914023012602</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2023.125509" target="_blank" >10.1016/j.talanta.2023.125509</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Impedimetric detection of gut-derived metabolites using 2D Germanene-based materials

Popis výsledku v původním jazyce

Apart from the extensively researched graphene under the Group 14 2D materials, monolayered germanene and its derivatives have been gaining interest lately as alternative class of 2D materials owing to their facile synthesis, and attractive electronic and optical properties. Herein, three different functionalized germanene-based nanomaterials, namely Ge–H, Ge-CH3 and Ge–C3-CN were investigated on their novel incorporation in impedimetric immunosensors for the detection of gut-derived metabolites associated with neurological diseases, such as kynurenic acid (KA) and quinolinic acid (QA). The designed germanene-based immunosensor relies on an indirect competitive mechanism using disposable electrode printed chips. The competition for a fixed binding site of a primary antibody occurs between the bovine serum albumin-conjugated antigens on the electrode surface and the free antigens in the solution. Among the three materials, Ge–H displayed superior bioanalytical performance in KA and QA detection. Lower limits of detection of 5.07–11.38 ng/mL (26.79–68.11 nM) were attained for KA and QA with a faster reaction time than previously reported methods. Also, minimal cross-reactivity with interfering compounds, good reproducibility in impedimetric responses (RSD = 2.43–7.51 %) and long-term stability up to a month at 4 °C were the other attributes that the proposed Ge–H competitive impedimetric immunosensor has accomplished. The application of the developed Ge–H immunosensor to serum samples allowed an accurate KA and QA quantification at physiologically relevant levels. This work serves as a stepping-stone in the development of germanene-based nanomaterials for their implementation into cost-effective, miniaturized, portable and rapid impedimetric immunosensors, which are highly desirable for point-of-care testing in clinical settings.

Název v anglickém jazyce

Impedimetric detection of gut-derived metabolites using 2D Germanene-based materials

Popis výsledku anglicky

Apart from the extensively researched graphene under the Group 14 2D materials, monolayered germanene and its derivatives have been gaining interest lately as alternative class of 2D materials owing to their facile synthesis, and attractive electronic and optical properties. Herein, three different functionalized germanene-based nanomaterials, namely Ge–H, Ge-CH3 and Ge–C3-CN were investigated on their novel incorporation in impedimetric immunosensors for the detection of gut-derived metabolites associated with neurological diseases, such as kynurenic acid (KA) and quinolinic acid (QA). The designed germanene-based immunosensor relies on an indirect competitive mechanism using disposable electrode printed chips. The competition for a fixed binding site of a primary antibody occurs between the bovine serum albumin-conjugated antigens on the electrode surface and the free antigens in the solution. Among the three materials, Ge–H displayed superior bioanalytical performance in KA and QA detection. Lower limits of detection of 5.07–11.38 ng/mL (26.79–68.11 nM) were attained for KA and QA with a faster reaction time than previously reported methods. Also, minimal cross-reactivity with interfering compounds, good reproducibility in impedimetric responses (RSD = 2.43–7.51 %) and long-term stability up to a month at 4 °C were the other attributes that the proposed Ge–H competitive impedimetric immunosensor has accomplished. The application of the developed Ge–H immunosensor to serum samples allowed an accurate KA and QA quantification at physiologically relevant levels. This work serves as a stepping-stone in the development of germanene-based nanomaterials for their implementation into cost-effective, miniaturized, portable and rapid impedimetric immunosensors, which are highly desirable for point-of-care testing in clinical settings.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/GX19-26910X" target="_blank" >GX19-26910X: Chemie ve dvou dimenzích - za hranice grafenu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TALANTA

ISSN

0039-9140

e-ISSN

1873-3573

Svazek periodika

270

Číslo periodika v rámci svazku

1 April 2024

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85180537805