The development of a butyrylcholinesterase porous pellet for innovative detection of cholinesterase inhibitors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F17%3A43913512" target="_blank" >RIV/60461373:22320/17:43913512 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16370/17:43875604

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ejps.2017.09.015" target="_blank" >http://dx.doi.org/10.1016/j.ejps.2017.09.015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejps.2017.09.015" target="_blank" >10.1016/j.ejps.2017.09.015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The development of a butyrylcholinesterase porous pellet for innovative detection of cholinesterase inhibitors

Popis výsledku v původním jazyce

The aim of the presented research was the preparation of an innovative carrier with significantly improved properties for the fast and sensitive detection of cholinesterase inhibitors such as nerve agents. This innovative carrier was in the form of spherical pellets containing different amounts of Neusilin. Neusilin is a synthetic and amorphous form of magnesium aluminometasilicate with a high specific surface area, and the immobilized enzyme butyrylcholinesterase with an activity of 50 nkat.g(-1). Pellets were prepared by the extrusion-spheronization method and dried in a hot air oven under two conditions - at 30 degrees C for 72 h and at 60 degrees C for 24 h. Dried pellets were consequently impregnated with a solution containing butyrylcholinesterase. Impregnated pellets were evaluated for their quality parameters, enzymatic activity and inhibition. Activity and inhibition were tested according to the standard Ellman&apos;s method. It was observed that the addition of Neusilin significantly increased the hardness, intraparticular porosity, sphericity and activity of the carriers as well as intensity of the color transition. Therefore it is shown that these carriers have unquestionable advantages over common carriers of their kind. Drying temperatures have been shown to have no effect on properties of pellets except for a change in their size. Results were confirmed by statistical evaluation using ANOVA and PCA.

Název v anglickém jazyce

The development of a butyrylcholinesterase porous pellet for innovative detection of cholinesterase inhibitors

Popis výsledku anglicky

The aim of the presented research was the preparation of an innovative carrier with significantly improved properties for the fast and sensitive detection of cholinesterase inhibitors such as nerve agents. This innovative carrier was in the form of spherical pellets containing different amounts of Neusilin. Neusilin is a synthetic and amorphous form of magnesium aluminometasilicate with a high specific surface area, and the immobilized enzyme butyrylcholinesterase with an activity of 50 nkat.g(-1). Pellets were prepared by the extrusion-spheronization method and dried in a hot air oven under two conditions - at 30 degrees C for 72 h and at 60 degrees C for 24 h. Dried pellets were consequently impregnated with a solution containing butyrylcholinesterase. Impregnated pellets were evaluated for their quality parameters, enzymatic activity and inhibition. Activity and inhibition were tested according to the standard Ellman&apos;s method. It was observed that the addition of Neusilin significantly increased the hardness, intraparticular porosity, sphericity and activity of the carriers as well as intensity of the color transition. Therefore it is shown that these carriers have unquestionable advantages over common carriers of their kind. Drying temperatures have been shown to have no effect on properties of pellets except for a change in their size. Results were confirmed by statistical evaluation using ANOVA and PCA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30100 - Basic medicine

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Pharmaceutical Sciences

ISSN

0928-0987

e-ISSN

—

Svazek periodika

109

Číslo periodika v rámci svazku

2017

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

548-555

Kód UT WoS článku

000413325000057

EID výsledku v databázi Scopus

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