Tubes for detection of cholinesterase inhibitors - unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00504873" target="_blank" >RIV/61389013:_____/19:00504873 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/19:00340441 RIV/62157124:16370/19:43877743

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0141022919300699?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0141022919300699?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.enzmictec.2019.05.002" target="_blank" >10.1016/j.enzmictec.2019.05.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tubes for detection of cholinesterase inhibitors - unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers

Popis výsledku v původním jazyce

Detection tubes are small devices for the colorimetric enzymatic detection of cholinesterase inhibitors such as sarin, soman, VX nerve agents and substances denoted as Novichok. These detectors contain carriers in the form of pellets with immobilized cholinesterase, substrate and detection reagent. Their advantages are portability, sensitivity and simplicity, enabling fast detection of such compounds from air and water in case of a terrorist attack or war. In general, maintaining the stability of an enzyme for a longer time is very problematic. Therefore, its further enhancement is required for safety and financial reasons. In this study, the stability of our patented carriers in the form of pellets with immobilized butyrylcholinesterase containing an increasing amount of the unique sorbent Neusilin® US2 was evaluated. The samples containing Neusilin maintained the stability of the immobilized enzyme for a longer time even at higher temperature and humidity than the currently commercially used carrier without Neusilin, allowing improved detection of nerve agents.

Název v anglickém jazyce

Tubes for detection of cholinesterase inhibitors - unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers

Popis výsledku anglicky

Detection tubes are small devices for the colorimetric enzymatic detection of cholinesterase inhibitors such as sarin, soman, VX nerve agents and substances denoted as Novichok. These detectors contain carriers in the form of pellets with immobilized cholinesterase, substrate and detection reagent. Their advantages are portability, sensitivity and simplicity, enabling fast detection of such compounds from air and water in case of a terrorist attack or war. In general, maintaining the stability of an enzyme for a longer time is very problematic. Therefore, its further enhancement is required for safety and financial reasons. In this study, the stability of our patented carriers in the form of pellets with immobilized butyrylcholinesterase containing an increasing amount of the unique sorbent Neusilin® US2 was evaluated. The samples containing Neusilin maintained the stability of the immobilized enzyme for a longer time even at higher temperature and humidity than the currently commercially used carrier without Neusilin, allowing improved detection of nerve agents.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Enzyme and Microbial Technology

ISSN

0141-0229

e-ISSN

—

Svazek periodika

128

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

26-33

Kód UT WoS článku

000472988700004

EID výsledku v databázi Scopus

2-s2.0-85065427273