Outstanding enrichment of ladderane lipids in anammox bacteria: overlooked effect of pH

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F24%3A43930173" target="_blank" >RIV/60461373:22320/24:43930173 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/24:43930173 RIV/60461373:22340/24:43930173

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0301479724039483?utm_campaign=STMJ_219742_AUTH_SERV_PA&utm_medium=email&utm_acid=96783346&SIS_ID=&dgcid=STMJ_219742_AUTH_SERV_PA&CMX_ID=&utm_in=DM533665&utm_source=AC_" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0301479724039483?utm_campaign=STMJ_219742_AUTH_SERV_PA&utm_medium=email&utm_acid=96783346&SIS_ID=&dgcid=STMJ_219742_AUTH_SERV_PA&CMX_ID=&utm_in=DM533665&utm_source=AC_</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jenvman.2024.123961" target="_blank" >10.1016/j.jenvman.2024.123961</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Outstanding enrichment of ladderane lipids in anammox bacteria: overlooked effect of pH

Popis výsledku v původním jazyce

Ladderane lipids synthesised by anammox bacteria hold significant potential for applications in jet fuel, drug delivery, and optoelectronics. Despite the widespread use of anammox bacteria in nitrogen removal from wastewater, the optimal conditions for maximising ladderane production remain unclear, limiting their broader application. To address this, we operated a fed-batch bioreactor with anammox bacteria, gradually adjusting the pH from 6.5 to 7.5 while regularly sampling for microbial community composition (Illumina sequencing), proteins, and ladderane lipids (UHPLC-HRMS). Our findings reveal that ladderane production positively correlates with rising pH increasing nearly fivefold as pH rose from 6.5 to 7.5, with a notable shift towards lipids containing two ladderane alkyl chains at higher pH. However, the conditions at an alkaline pH range also induced mild stress in anammox bacteria, as evidenced by our proteomic and microbial community data. Therefore, we propose maintaining a pH above 7.5 to enrich ladderane-rich anammox biomass but emphasise the need forgradual adaptation. This approach could optimise anammox installations for producing high-value ladderane lipids from wastewater.

Název v anglickém jazyce

Outstanding enrichment of ladderane lipids in anammox bacteria: overlooked effect of pH

Popis výsledku anglicky

Ladderane lipids synthesised by anammox bacteria hold significant potential for applications in jet fuel, drug delivery, and optoelectronics. Despite the widespread use of anammox bacteria in nitrogen removal from wastewater, the optimal conditions for maximising ladderane production remain unclear, limiting their broader application. To address this, we operated a fed-batch bioreactor with anammox bacteria, gradually adjusting the pH from 6.5 to 7.5 while regularly sampling for microbial community composition (Illumina sequencing), proteins, and ladderane lipids (UHPLC-HRMS). Our findings reveal that ladderane production positively correlates with rising pH increasing nearly fivefold as pH rose from 6.5 to 7.5, with a notable shift towards lipids containing two ladderane alkyl chains at higher pH. However, the conditions at an alkaline pH range also induced mild stress in anammox bacteria, as evidenced by our proteomic and microbial community data. Therefore, we propose maintaining a pH above 7.5 to enrich ladderane-rich anammox biomass but emphasise the need forgradual adaptation. This approach could optimise anammox installations for producing high-value ladderane lipids from wastewater.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

<a href="/cs/project/GA20-13766S" target="_blank" >GA20-13766S: Unikátní ladderánové fosfolipidy v bakteriích anammox: potenciální cenný produkt z odpadních vod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Environmental Management

ISSN

0301-4797

e-ISSN

1095-8630

Svazek periodika

2024

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

123961-123973

Kód UT WoS článku

001423758000001

EID výsledku v databázi Scopus

2-s2.0-85213493574