Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F25%3A43930500" target="_blank" >RIV/60461373:22320/25:43930500 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/25:43930500

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2213343725003239" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2213343725003239</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jece.2025.115628" target="_blank" >10.1016/j.jece.2025.115628</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Popis výsledku v původním jazyce

Although anammox-based processes have been widely applied in mesophilic conditions of reject water and recently in mainstream conditions, the potential of their implementation in high-temperature wastewaters remains largely unexplored. Therefore, this study investigated the operation parameters for the successful adaptation of anammox bacteria and the mechanisms involved at the proteomic and cellular levels, including unique ladderane lipids. For this purpose, ‘Candidatus Brocadia’ enrichment was cultivated in two fed-batch reactors (FBRs) at a lab scale. The temperature of one FBR was gradually increased from 30 to 40 °C while the other FBR was maintained at 30 °C with four consecutive replicates of this experiment. To ensure successful adaptation, the original loading rate needed to be reduced by at least half, or preferably kept below 50 % of the specific anammox activity level at that time. The most notable adaptation mechanisms included: (1) upregulation of chaperones and (2) doubled ladderane cyclization via the replacement of nonladderane fatty acid by a ladderane fatty acid in ladderane lipids (p-value 0.005). To the best of our knowledge, this is the first study to describe the novel mechanism of ladderane cyclization, which, together with other adaptation strategies, presents crucial indicators in anammox adaptation to high-temperature wastewaters.

Název v anglickém jazyce

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Popis výsledku anglicky

Although anammox-based processes have been widely applied in mesophilic conditions of reject water and recently in mainstream conditions, the potential of their implementation in high-temperature wastewaters remains largely unexplored. Therefore, this study investigated the operation parameters for the successful adaptation of anammox bacteria and the mechanisms involved at the proteomic and cellular levels, including unique ladderane lipids. For this purpose, ‘Candidatus Brocadia’ enrichment was cultivated in two fed-batch reactors (FBRs) at a lab scale. The temperature of one FBR was gradually increased from 30 to 40 °C while the other FBR was maintained at 30 °C with four consecutive replicates of this experiment. To ensure successful adaptation, the original loading rate needed to be reduced by at least half, or preferably kept below 50 % of the specific anammox activity level at that time. The most notable adaptation mechanisms included: (1) upregulation of chaperones and (2) doubled ladderane cyclization via the replacement of nonladderane fatty acid by a ladderane fatty acid in ladderane lipids (p-value 0.005). To the best of our knowledge, this is the first study to describe the novel mechanism of ladderane cyclization, which, together with other adaptation strategies, presents crucial indicators in anammox adaptation to high-temperature wastewaters.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

<a href="/cs/project/GA20-13766S" target="_blank" >GA20-13766S: Unikátní ladderánové fosfolipidy v bakteriích anammox: potenciální cenný produkt z odpadních vod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Environmental Chemical Engineering

ISSN

2213-2929

e-ISSN

2213-3437

Svazek periodika

13

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

115628-115637

Kód UT WoS článku

001434268800001

EID výsledku v databázi Scopus

2-s2.0-85216659765