Developmnet of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008357" target="_blank" >RIV/60461373:22330/03:00008357 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Developmnet of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples

Popis výsledku v původním jazyce

Two enzyme-linked immunosorbent assays (ELISA) with chemiluminescent (CL) detection for the insecticide DDT and the group of DDT-related compounds have been optimized and characterized. Both conjugate-coated ELISAs are based on monoclonal antibodies (MAbs) of different specificity and homologous protein conjugates. Effects of several physicochemical factors (ionic strength, pH, Tween-20 and Bovine serum albumin (BSA) concentrations) and solvents (methanol, ethanol, acetone and N,N'-dimethylformamide) onthe performance of the assays were studied and optimized. For the DDT-selective assay, the sensitivity, estimated as the I-50 value, was 0.6 mug/l, with a linear working range between 0.1 and 2 mug/l and a limit of detection of 0.06 mug/l. For the DDT group-selective assay, the sensitivity was 0.2 mug/l, with a linear working range between 0.07 and 1 mug/l and a limit of detection of 0.04 mug/l. CL-ELISAs were four times more sensitive compared to colorimetric ELISAs. Finally, both immu

Název v anglickém jazyce

Developmnet of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples

Popis výsledku anglicky

Two enzyme-linked immunosorbent assays (ELISA) with chemiluminescent (CL) detection for the insecticide DDT and the group of DDT-related compounds have been optimized and characterized. Both conjugate-coated ELISAs are based on monoclonal antibodies (MAbs) of different specificity and homologous protein conjugates. Effects of several physicochemical factors (ionic strength, pH, Tween-20 and Bovine serum albumin (BSA) concentrations) and solvents (methanol, ethanol, acetone and N,N'-dimethylformamide) onthe performance of the assays were studied and optimized. For the DDT-selective assay, the sensitivity, estimated as the I-50 value, was 0.6 mug/l, with a linear working range between 0.1 and 2 mug/l and a limit of detection of 0.06 mug/l. For the DDT group-selective assay, the sensitivity was 0.2 mug/l, with a linear working range between 0.07 and 1 mug/l and a limit of detection of 0.04 mug/l. CL-ELISAs were four times more sensitive compared to colorimetric ELISAs. Finally, both immu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF IMMUNOLOGICAL METHODS

ISSN

0022-1759

e-ISSN

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Svazek periodika

283

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

45-57

Kód UT WoS článku

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EID výsledku v databázi Scopus

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