Stanovení N-methylkarbamátových pesticidů pomocí enzymové imunoanalýzy s chemiluminescenční detekcí

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012904" target="_blank" >RIV/60461373:22330/04:00012904 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Determination of N-methylcarbamate Pesticides Using Enzyme Immunoassays with Chemiluminescent Detection

Popis výsledku v původním jazyce

In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAswith colorimetric detection. The sensitivity of immunochemical methods was expressed as detection limit, linear working range, and I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease the optimal antibody and conjugate concentrations and to reach better analytical parameters. The experimental comparison of the analytical performance of the ELISAs was carried out by analysing simply diluted fruit juices, spiked at different concentration levels with the above mentioned pesticides. Recovery values for both ELISAs were around 100 % and no matrix effects were observed when fruit juices were diluted 1:20 or more.

Název v anglickém jazyce

The Determination of N-methylcarbamate Pesticides Using Enzyme Immunoassays with Chemiluminescent Detection

Popis výsledku anglicky

In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAswith colorimetric detection. The sensitivity of immunochemical methods was expressed as detection limit, linear working range, and I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease the optimal antibody and conjugate concentrations and to reach better analytical parameters. The experimental comparison of the analytical performance of the ELISAs was carried out by analysing simply diluted fruit juices, spiked at different concentration levels with the above mentioned pesticides. Recovery values for both ELISAs were around 100 % and no matrix effects were observed when fruit juices were diluted 1:20 or more.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Czech Journal of Food Science

ISBN

1212-1800

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

280-282

Název nakladatele

Česká akademie zemědělských věd

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

29. 9. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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