Development of an indirect competitive enzyme-linked immunosorbent assay of DDT

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008938" target="_blank" >RIV/60461373:22330/03:00008938 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of an indirect competitive enzyme-linked immunosorbent assay of DDT

Popis výsledku v původním jazyce

Indirect competitive enzyme immunoassays of DDT based on polyclonal antibodies have been developed. The limit of detection and limit of quantification were 0.3 nmol l-1 and 4.2 nmol l-1, respectively. Non-specific interaction was eliminated by the saturation of the solid phase with 2 % human serum albumin. Different polystyrene microplates were tested. The cross-reactivity of the polyclonal rabbit antibody to DDT with 2,4'-DDE, 4,4'-DDD, 2,4'-DDT, 2,4'-DDD, Methoxychlor and 4,4'-DDE was 1 %, 9 %, 12 %,13 %, 36 % and 186 %, respectively. No cross-reactivity of the tested antibody with 4,4'-DDA, 4,4'-DDOH, 4,4'-DBP, 4,4'-DDM, 4,4'-PCB, 2,3'-PCB, 4-PCB and 3-PCB was observed. With respect to a limited solubility of the analyte in water, the influence ofdifferent concentrations of methanol, ethanol, acetone and N,N'-dimethylformamide on the assay sensitivity were tested. A recommended concentration of the organic solvents is 5-10 % (v/v) in the reaction mixture. Preliminary tests were do

Název v anglickém jazyce

Development of an indirect competitive enzyme-linked immunosorbent assay of DDT

Popis výsledku anglicky

Indirect competitive enzyme immunoassays of DDT based on polyclonal antibodies have been developed. The limit of detection and limit of quantification were 0.3 nmol l-1 and 4.2 nmol l-1, respectively. Non-specific interaction was eliminated by the saturation of the solid phase with 2 % human serum albumin. Different polystyrene microplates were tested. The cross-reactivity of the polyclonal rabbit antibody to DDT with 2,4'-DDE, 4,4'-DDD, 2,4'-DDT, 2,4'-DDD, Methoxychlor and 4,4'-DDE was 1 %, 9 %, 12 %,13 %, 36 % and 186 %, respectively. No cross-reactivity of the tested antibody with 4,4'-DDA, 4,4'-DDOH, 4,4'-DBP, 4,4'-DDM, 4,4'-PCB, 2,3'-PCB, 4-PCB and 3-PCB was observed. With respect to a limited solubility of the analyte in water, the influence ofdifferent concentrations of methanol, ethanol, acetone and N,N'-dimethylformamide on the assay sensitivity were tested. A recommended concentration of the organic solvents is 5-10 % (v/v) in the reaction mixture. Preliminary tests were do

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F02%2F0287" target="_blank" >GA525/02/0287: Vývoj screeningových metod ELISA a PCR pro detekci bakterií rodu Campylobacter v potravinách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food and Agricultural Immunology

ISSN

0954-0105

e-ISSN

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Svazek periodika

14

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

285-300

Kód UT WoS článku

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EID výsledku v databázi Scopus

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