Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008962" target="_blank" >RIV/60461373:22330/03:00008962 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník

Popis výsledku v původním jazyce

Phospholipid signalling pathway is one of the basic signalling mechanism in animal as well as plant cells. Phospholipases and phospholipids are the key components of this system. Phospholipases are divided based on the site of cleavage of the phospholipid molecules to phospholipase A2, C and D. The aim of this study was to established sophisticated method which let us to analyse products of signalling phospholipases in its entirety with one method. Phospholipid signalling pathway was studied insitu in tobacco cell suspension culture BY-2 using different types of fluorescently labelled (BODIPY) phosphatidylcholine. Incorporation of the fluorescent substrates to tobacco cell membranes was monitored by fluorescent microscopy and confirmed by pla smolysis.Fluorescent products of cleaved phosphatidylcholine were analysed by HP-TLC and newly developed HPLC methods. First the fluorescent standards of individual phospholipase products were prepared using commercial phospholipases and then use

Název v anglickém jazyce

Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník

Popis výsledku anglicky

Phospholipid signalling pathway is one of the basic signalling mechanism in animal as well as plant cells. Phospholipases and phospholipids are the key components of this system. Phospholipases are divided based on the site of cleavage of the phospholipid molecules to phospholipase A2, C and D. The aim of this study was to established sophisticated method which let us to analyse products of signalling phospholipases in its entirety with one method. Phospholipid signalling pathway was studied insitu in tobacco cell suspension culture BY-2 using different types of fluorescently labelled (BODIPY) phosphatidylcholine. Incorporation of the fluorescent substrates to tobacco cell membranes was monitored by fluorescent microscopy and confirmed by pla smolysis.Fluorescent products of cleaved phosphatidylcholine were analysed by HP-TLC and newly developed HPLC methods. First the fluorescent standards of individual phospholipase products were prepared using commercial phospholipases and then use

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F03%2F0353" target="_blank" >GA522/03/0353: Mechanismy podílející se na obranné reakci řepky (Brassica napus) vůči pathogenům a vzniku indukované resistence</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

FEBS Advanced Course: Lipid signalling and membrane traffic

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

99

Název nakladatele

Advanced FEBS Course

Místo vydání

Itálie

Místo konání akce

Santa Mario Imbaro

Datum konání akce

20. 6. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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