Cloning of a bacterial PCB-degrading gene into the plants of Nicotiana tabacum - sborník

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008981" target="_blank" >RIV/60461373:22330/03:00008981 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cloning of a bacterial PCB-degrading gene into the plants of Nicotiana tabacum - sborník

Popis výsledku v původním jazyce

The target of this work is to increase biodegradation of polychlorinated biphenyls (PCB) by plants transformed with the gene bph C originally located in genome of PCB degrading bacteria Comamonas testosteroni B356. The gene bphC encoding the enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase was cloned in cassette first with the detection marker GFP (green fluorescent protein) to plant cells of Nicotiana tabacum. Transgenic plants proved higher resistence to PCB than nontrasgenic ones. Because of the difficulties with the detection of GFP in plants, caused by autofluorescence of plant tissue, also other constructs were prepared. These contain luciferase gene cloned next to the bphC gene, beta-glucuronidase gene or six histidine motives. Histidine tail allowsfacility isolation of the enzyme. The presence of bphC/GFP DNA and RNA was proved by PCR using various sets of primers. In some of the transformants the fusion protein bphC/GFP was detected by Western blot analysis. Other constructs with

Název v anglickém jazyce

Cloning of a bacterial PCB-degrading gene into the plants of Nicotiana tabacum - sborník

Popis výsledku anglicky

The target of this work is to increase biodegradation of polychlorinated biphenyls (PCB) by plants transformed with the gene bph C originally located in genome of PCB degrading bacteria Comamonas testosteroni B356. The gene bphC encoding the enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase was cloned in cassette first with the detection marker GFP (green fluorescent protein) to plant cells of Nicotiana tabacum. Transgenic plants proved higher resistence to PCB than nontrasgenic ones. Because of the difficulties with the detection of GFP in plants, caused by autofluorescence of plant tissue, also other constructs were prepared. These contain luciferase gene cloned next to the bphC gene, beta-glucuronidase gene or six histidine motives. Histidine tail allowsfacility isolation of the enzyme. The presence of bphC/GFP DNA and RNA was proved by PCR using various sets of primers. In some of the transformants the fusion protein bphC/GFP was detected by Western blot analysis. Other constructs with

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA526%2F01%2F1292" target="_blank" >GA526/01/1292: Studium metabolismu a vztahu rhizosferních mikroorganismů s rostlinnými systémy při odbourávání organických sloučenin kontaminujících životní prostředí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceeding of 6th International Symposium and Exhibition on Environmental Contamination in Central and Eastern Europe

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

175

Název nakladatele

Florida State University

Místo vydání

Florida

Místo konání akce

Praha

Datum konání akce

1. 9. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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