Vývoj monoklonální imunonometody selektivní pro chlorované cyklodienové insekticidy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012768" target="_blank" >RIV/60461373:22330/04:00012768 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of monoclonal immunoassay selective for chlorinated cyclodiene insecticides

Popis výsledku v původním jazyce

Organochlorine pesticides still generate public health concerns because of their unresolved health impact and their persistence in living beings, which is demanding appropriate analytical techniques for their monitoring. In this study, an enzyme-linked immunosorbent assay based on monoclonal antibodies (MAbs) for the detection of an important group of organochlorine pesticides, the cyclodiene group, has been developed. With this aim, several hapten-protein conjugates, characterized by exposure of the common hexachlorinated bicyclic (norbornene) moiety and differing in the linking structure to the carrier protein, were prepared. From mice immunized with these conjugates, several MAbs with the ability to sensitively bind the majority of cyclodienes wereobtained. Among them CCD2.2 MAb displaying the broadest recognition to cyclodiene compounds (endosulfan, dieldrin, endrin, chlordane, heptachlor, aldrin, toxaphene: I50 values in the 6-25 nM range) was selected for the assay. Interestingl

Název v anglickém jazyce

Development of monoclonal immunoassay selective for chlorinated cyclodiene insecticides

Popis výsledku anglicky

Organochlorine pesticides still generate public health concerns because of their unresolved health impact and their persistence in living beings, which is demanding appropriate analytical techniques for their monitoring. In this study, an enzyme-linked immunosorbent assay based on monoclonal antibodies (MAbs) for the detection of an important group of organochlorine pesticides, the cyclodiene group, has been developed. With this aim, several hapten-protein conjugates, characterized by exposure of the common hexachlorinated bicyclic (norbornene) moiety and differing in the linking structure to the carrier protein, were prepared. From mice immunized with these conjugates, several MAbs with the ability to sensitively bind the majority of cyclodienes wereobtained. Among them CCD2.2 MAb displaying the broadest recognition to cyclodiene compounds (endosulfan, dieldrin, endrin, chlordane, heptachlor, aldrin, toxaphene: I50 values in the 6-25 nM range) was selected for the assay. Interestingl

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Agricultural and Food Chemistry

ISSN

0021-8561

e-ISSN

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Svazek periodika

52

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

2776-2784

Kód UT WoS článku

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EID výsledku v databázi Scopus

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