ELISA pro stanovení isoflavonů v potravinářsky významných rostlinách

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012778" target="_blank" >RIV/60461373:22330/04:00012778 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants

Popis výsledku v původním jazyce

Isoflavones are phytoestrogens occuring chiefly in leguminous plants, however they were reported also in certain non-leguminous species. They were shown to influence steroid metabolizing enzymes and estrogen receptors in plant consuming animals and are thought to be capable of modifying the estrogen action in woman. The other effects on humans are the antioxidant activity, moderation of menopausal symptoms and prevention of several types of cancer (e.g. prostate and breast cancers), which are increasingly investigated in recent years. In this work, the development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin, is presented. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4' via a carboxy methyl spacer. The developed ELISAs are highly specific, I50 values of the standard curves range between 0,1-2 ng/ml. The cross re

Název v anglickém jazyce

Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants

Popis výsledku anglicky

Isoflavones are phytoestrogens occuring chiefly in leguminous plants, however they were reported also in certain non-leguminous species. They were shown to influence steroid metabolizing enzymes and estrogen receptors in plant consuming animals and are thought to be capable of modifying the estrogen action in woman. The other effects on humans are the antioxidant activity, moderation of menopausal symptoms and prevention of several types of cancer (e.g. prostate and breast cancers), which are increasingly investigated in recent years. In this work, the development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin, is presented. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4' via a carboxy methyl spacer. The developed ELISAs are highly specific, I50 values of the standard curves range between 0,1-2 ng/ml. The cross re

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Czech Journal of Food Science

ISBN

1212-1800

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

199-202

Název nakladatele

Česká akademie zemědělských věd

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

29. 9. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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