Plasmid pA81 z kmene Achromobacter xylosoxidans A8, který degraduje chlorbenzoáty

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012857" target="_blank" >RIV/60461373:22330/04:00012857 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Plasmid pA81 from chlorobenzoate degrading strain Achromobacter xylosoxidans A8

Popis výsledku v původním jazyce

The Achromobacter xylosoxidans strain A8 was isolated in the Czech Republic from PCB contaminated soil. It is able to use 2-clorobenzoate (2-CB) and 2,5-dichlorobenzoate (2,5-DCB) as sole sources of carbon and energy. We analyze the A. xylosoxidans A8 with the aim to characterize genes involved in the degradation of CBs.The genome of this strain contains two large conjugative plasmids pA81 (~ 100 kbp) and pA82 (~ 250 kbp). The genes encoding enzymes involved in the degradation pathway were found to be situated directly on the plasmid pA81. On the basis of the pA81 sequence analysis we identified clusters responsible for the plasmid replication, conjugative transfer, stable inheritance, degradation of xenobiotics and transposition. Comparison of the plasmid "backbone" constituted by regions of replication control and stability and two Tra1/Tra2 regions responsible for conjugative transfer shows the highest similarity to the backbone of IncPß plasmids. Members of this plasmid group can r

Název v anglickém jazyce

Plasmid pA81 from chlorobenzoate degrading strain Achromobacter xylosoxidans A8

Popis výsledku anglicky

The Achromobacter xylosoxidans strain A8 was isolated in the Czech Republic from PCB contaminated soil. It is able to use 2-clorobenzoate (2-CB) and 2,5-dichlorobenzoate (2,5-DCB) as sole sources of carbon and energy. We analyze the A. xylosoxidans A8 with the aim to characterize genes involved in the degradation of CBs.The genome of this strain contains two large conjugative plasmids pA81 (~ 100 kbp) and pA82 (~ 250 kbp). The genes encoding enzymes involved in the degradation pathway were found to be situated directly on the plasmid pA81. On the basis of the pA81 sequence analysis we identified clusters responsible for the plasmid replication, conjugative transfer, stable inheritance, degradation of xenobiotics and transposition. Comparison of the plasmid "backbone" constituted by regions of replication control and stability and two Tra1/Tra2 regions responsible for conjugative transfer shows the highest similarity to the backbone of IncPß plasmids. Members of this plasmid group can r

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/LN00A079" target="_blank" >LN00A079: Centrum integrované genomiky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the XIX. meeting of czech and slovak societies for biochemistry and molecula r biology

ISBN

0232-0061/80-24

ISSN

—

e-ISSN

—

Počet stran výsledku

1

Strana od-do

228

Název nakladatele

Univerzita Palackého

Místo vydání

Olomouc

Místo konání akce

Olomouc

Datum konání akce

1. 1. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—