Porovnání konvenční HPLC/MS s moderní UPLC/MS v analýze 67 polárních pesticidů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00015505" target="_blank" >RIV/60461373:22330/05:00015505 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

COMPARISON CONVENTIONAL HPLC/MS WITH UP-TODAY UPLC/MS METHOD FOR ANALYSIS OF 67 POLAR PESTICIDES

Popis výsledku v původním jazyce

The up-today UPLC (Ultra Performance Liquid Chromatography) Acquity systém serves a lot of advantages in the area of polar pesticide analysis. The Waters Acquity system works at high pressures (up to 15.000 psi) with BEH columns. Thanks to these chromatographic parameters, providing high resolution that allows faster separation. For determination of 67 polar pesticides it is commonly used HPLC/MS Waters Aliance 2695 / Quattro Premier. This analytical method is accredited (ISO 17025) and detections limits (LOD) of all analytes are under the hygienic limits for baby food (MRL=0.010 mg/kg). For separation Supelco Discovery C-18 (150 mm, 3 mm, 5 mm) was used with mobile phase gradient of 0.01M ammonium acetate in water/MetOH and flow rate 0.3 ml/min. The analysis is finished in 50 min, so within 24 hours 16 samples can by analyzed including calibration curves and QC samples. This method was transferred and optimized on Waters UPLC Acquity system coupled with Waters Quattro Premier. For sep

Název v anglickém jazyce

COMPARISON CONVENTIONAL HPLC/MS WITH UP-TODAY UPLC/MS METHOD FOR ANALYSIS OF 67 POLAR PESTICIDES

Popis výsledku anglicky

The up-today UPLC (Ultra Performance Liquid Chromatography) Acquity systém serves a lot of advantages in the area of polar pesticide analysis. The Waters Acquity system works at high pressures (up to 15.000 psi) with BEH columns. Thanks to these chromatographic parameters, providing high resolution that allows faster separation. For determination of 67 polar pesticides it is commonly used HPLC/MS Waters Aliance 2695 / Quattro Premier. This analytical method is accredited (ISO 17025) and detections limits (LOD) of all analytes are under the hygienic limits for baby food (MRL=0.010 mg/kg). For separation Supelco Discovery C-18 (150 mm, 3 mm, 5 mm) was used with mobile phase gradient of 0.01M ammonium acetate in water/MetOH and flow rate 0.3 ml/min. The analysis is finished in 50 min, so within 24 hours 16 samples can by analyzed including calibration curves and QC samples. This method was transferred and optimized on Waters UPLC Acquity system coupled with Waters Quattro Premier. For sep

Druh

O - Ostatní výsledky

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů