Stanovení hlavních trichothecenových mykotoxinů a zearalenonu v pšenici pomocí vysokotlaké extrakce rozpouštědlem a vysokoúčinné kapalinové chromatografie ve spojení s tandemovou hmotnostní spektrometrií

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00015522" target="_blank" >RIV/60461373:22330/05:00015522 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DETERMINATION OF MAJOR TRICHOTHECENE MYCOTOXINS AND ZEARALENONE IN WHEAT USING PRESSURIZED SOLVENT EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

Popis výsledku v původním jazyce

Currently, deoxynivalenol (DON) and zearalenon are the only regulated Fusarium toxins in the Czech Republic. Recently, EFSA Scientific Committee proposed to extend the list of trichothecenes for which MRL is set. A novel method for determination of the trichothecenes nivalenol, deoxynivalenol, fusarenon-X, the sum of 3-acetyl-deoxynivalenol and 15-acetyldeoxynivalenol, HT-2 toxin, T-2 toxin and zearalenone in wheat has been developed using HPLC coupled with atmospheric pressure chemical ionization tandem mass spectrometry (LCMS/MS). Pressurized solvent extraction (PLE) was setted up as procedure for isolation of target compounds and its efficiency was compared with standardly used extraction (shaking with micture acetonitrile:water 84:16, v/v). Quick clean up of crude extract was performed with MycoSep# #226 or MycoSep# #225. Detection of target mycotoxins was carried out in the selected reaction monitoring (SRM) mode. The developed method was validated by use of spiked blank wheat sam

Název v anglickém jazyce

DETERMINATION OF MAJOR TRICHOTHECENE MYCOTOXINS AND ZEARALENONE IN WHEAT USING PRESSURIZED SOLVENT EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

Popis výsledku anglicky

Currently, deoxynivalenol (DON) and zearalenon are the only regulated Fusarium toxins in the Czech Republic. Recently, EFSA Scientific Committee proposed to extend the list of trichothecenes for which MRL is set. A novel method for determination of the trichothecenes nivalenol, deoxynivalenol, fusarenon-X, the sum of 3-acetyl-deoxynivalenol and 15-acetyldeoxynivalenol, HT-2 toxin, T-2 toxin and zearalenone in wheat has been developed using HPLC coupled with atmospheric pressure chemical ionization tandem mass spectrometry (LCMS/MS). Pressurized solvent extraction (PLE) was setted up as procedure for isolation of target compounds and its efficiency was compared with standardly used extraction (shaking with micture acetonitrile:water 84:16, v/v). Quick clean up of crude extract was performed with MycoSep# #226 or MycoSep# #225. Detection of target mycotoxins was carried out in the selected reaction monitoring (SRM) mode. The developed method was validated by use of spiked blank wheat sam

Druh

O - Ostatní výsledky

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů