Sledování aktivity kvasinek fluorescenční metodou

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00015695" target="_blank" >RIV/60461373:22330/05:00015695 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Yeast activity monitoring by fluorescent methods

Popis výsledku v původním jazyce

Yeast activity was monitored during pilot plant yeast propagation under aerobic and anaerobic conditions. The work was carried out with three widely used Czech lager yeast strains. Fluorescent intensity of DNA was analyzed by flow cytometry and signal was used as a marker for cell cycle determination. During experiments were monitored changes in significant properties of yeast (glycogen, proteins, and DNA) by fluorescent methods. It seemed that the impact of aerobic conditions on preservation of flocculation abilities depends on the genetic properties of each strain. Only one strain didn't show significant losses in flocculation abilities neither under aerobic nor strictly anaerobic conditions.

Název v anglickém jazyce

Yeast activity monitoring by fluorescent methods

Popis výsledku anglicky

Yeast activity was monitored during pilot plant yeast propagation under aerobic and anaerobic conditions. The work was carried out with three widely used Czech lager yeast strains. Fluorescent intensity of DNA was analyzed by flow cytometry and signal was used as a marker for cell cycle determination. During experiments were monitored changes in significant properties of yeast (glycogen, proteins, and DNA) by fluorescent methods. It seemed that the impact of aerobic conditions on preservation of flocculation abilities depends on the genetic properties of each strain. Only one strain didn't show significant losses in flocculation abilities neither under aerobic nor strictly anaerobic conditions.

Druh

O - Ostatní výsledky

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/1M0570" target="_blank" >1M0570: Výzkumné centrum pro studium obsahových látek ječmene a chmele</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů