Optimalizace metody pro stanovení polycyklických aromatických uhlovodíků v rybách; odhad nejistoty metody

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F06%3A00017805" target="_blank" >RIV/60461373:22330/06:00017805 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimisation of procedure for determination of polycyclic aromatic hydrocarbons and their derivatives in fish tissue: Estimation of measurements uncertainty

Popis výsledku v původním jazyce

Three alternative procedures were employed for isolation of polycyclic aromatic hydrocarbons (PAHs), their methyl-derivatives and sulphur analogues from fish tissue: (i) Soxhlet extraction, (ii) batch extraction enhanced by sonication and (iii) saponification of sample followed by reextraction of analytes into hexane. Soxhlet extraction using hexane-acetone (1:1, v/v) was found as the most efficient extraction technique, with analyte recoveries in the range 70-108 %. Within optimisation of clean-up step, several types of gel permeation chromatography (GPC) systems were tested: two types of polystyrene divinylbenzene copolymer gels (PSDVB), both "soft" gel type (Bio-Beads S-X3) and "rigid" gels type (PL gel and Envirogel) in combination with various mobile phases were compared. Bio-Beads S-X3 and mobile phase chloroform were found as the most appropriate for purifying of crude extracts prior to the final determinative step. High performance liquid chromatography with fluorimetric de

Název v anglickém jazyce

Optimisation of procedure for determination of polycyclic aromatic hydrocarbons and their derivatives in fish tissue: Estimation of measurements uncertainty

Popis výsledku anglicky

Three alternative procedures were employed for isolation of polycyclic aromatic hydrocarbons (PAHs), their methyl-derivatives and sulphur analogues from fish tissue: (i) Soxhlet extraction, (ii) batch extraction enhanced by sonication and (iii) saponification of sample followed by reextraction of analytes into hexane. Soxhlet extraction using hexane-acetone (1:1, v/v) was found as the most efficient extraction technique, with analyte recoveries in the range 70-108 %. Within optimisation of clean-up step, several types of gel permeation chromatography (GPC) systems were tested: two types of polystyrene divinylbenzene copolymer gels (PSDVB), both "soft" gel type (Bio-Beads S-X3) and "rigid" gels type (PL gel and Envirogel) in combination with various mobile phases were compared. Bio-Beads S-X3 and mobile phase chloroform were found as the most appropriate for purifying of crude extracts prior to the final determinative step. High performance liquid chromatography with fluorimetric de

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Additives and Contaminants

ISSN

0265-203X

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

309-325

Kód UT WoS článku

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EID výsledku v databázi Scopus

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