Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F09%3A00022373" target="_blank" >RIV/60461373:22330/09:00022373 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings

Popis výsledku v původním jazyce

The fractionation of Cd, Cu, Mo, Ni, and Zn species in extracts of rye (cv. Fernando) seedlings (grown up in both standard and Cd2+-enriched medium) and rye flour was performed by SEC/ICP-MS method. The majority of Cu, Zn, and Ni in all samples were bound in the 1-2 kDa fraction. Molybdenum occurred in all samples in the fraction of 3 kDa. During five days of cultivation in a solution of 30 ?mol/l Cd2+, the plants accumulated as much as 5 mg/kg fresh matter of Cd, but its soluble portion represented only 12-15%. The prevailing portion of Cd complexes was contained in the fraction of 3 kDa, while the minor part occurred in the fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level.The metal-rich fractions of the extracts of all samples (i.e. those of 1-2 kDa) were refined by immobilised metal affinity chromatography. The isolated ligands of trace elements were peptides rich in dicarboxylic aminoacids.

Název v anglickém jazyce

Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings

Popis výsledku anglicky

The fractionation of Cd, Cu, Mo, Ni, and Zn species in extracts of rye (cv. Fernando) seedlings (grown up in both standard and Cd2+-enriched medium) and rye flour was performed by SEC/ICP-MS method. The majority of Cu, Zn, and Ni in all samples were bound in the 1-2 kDa fraction. Molybdenum occurred in all samples in the fraction of 3 kDa. During five days of cultivation in a solution of 30 ?mol/l Cd2+, the plants accumulated as much as 5 mg/kg fresh matter of Cd, but its soluble portion represented only 12-15%. The prevailing portion of Cd complexes was contained in the fraction of 3 kDa, while the minor part occurred in the fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level.The metal-rich fractions of the extracts of all samples (i.e. those of 1-2 kDa) were refined by immobilised metal affinity chromatography. The isolated ligands of trace elements were peptides rich in dicarboxylic aminoacids.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Food Science

ISSN

1212-1800

e-ISSN

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Svazek periodika

27

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000264402600005

EID výsledku v databázi Scopus

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