Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F02%3A00007175" target="_blank" >RIV/60461373:22340/02:00007175 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/02:00007175

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS

Popis výsledku v původním jazyce

Soluble species of phosphorus, sulfur, selenium and eight metals (Mn, Fe, Co, Ni, Cu, Zn, Mo and Cd) in soybean flour and common bean seeds were investigated by size exclusion chromatography (SEC) and ICP-MS. Samples were extracted by 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5). Fractionation of sample extracts by preparative scale SEC was accomplished using Fractogel EMD BioSEC column (600 16 mm) and 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5) as a mobile phase (flow rate 2 ml min-1). 2 ml of sample were injected. Contents of elements in chromatographic fractions were determined by AAS, ICP-AES and ICP-MS. The elution profiles of P, Fe, Co, Ni, Cu, Zn and Mo in both samples were similar. Main species of Co, Ni, Cu, Zn and Mo were found in low molecular weight region (2 to 5 kDa), whereas Fe is predominantly bound to high molecular weight compounds (180 kDa). Dominant phosphorus fraction was detected in medium molecular weight region (10 to 30 kDa) and the other one in low molecu

Název v anglickém jazyce

Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS

Popis výsledku anglicky

Soluble species of phosphorus, sulfur, selenium and eight metals (Mn, Fe, Co, Ni, Cu, Zn, Mo and Cd) in soybean flour and common bean seeds were investigated by size exclusion chromatography (SEC) and ICP-MS. Samples were extracted by 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5). Fractionation of sample extracts by preparative scale SEC was accomplished using Fractogel EMD BioSEC column (600 16 mm) and 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5) as a mobile phase (flow rate 2 ml min-1). 2 ml of sample were injected. Contents of elements in chromatographic fractions were determined by AAS, ICP-AES and ICP-MS. The elution profiles of P, Fe, Co, Ni, Cu, Zn and Mo in both samples were similar. Main species of Co, Ni, Cu, Zn and Mo were found in low molecular weight region (2 to 5 kDa), whereas Fe is predominantly bound to high molecular weight compounds (180 kDa). Dominant phosphorus fraction was detected in medium molecular weight region (10 to 30 kDa) and the other one in low molecu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/GA525%2F00%2F0581" target="_blank" >GA525/00/0581: Speciační analýza stopových prvků v potravinách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography B

ISSN

1570-0232

e-ISSN

—

Svazek periodika

770

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

261-273

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—