Stanovení lamotriginu v séru pomocí on-line spojení kapilární isotachoforesy a kapilární zónové elektroforesy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F09%3A00022426" target="_blank" >RIV/60461373:22330/09:00022426 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Stanovení lamotriginu v séru pomocí on-line spojení kapilární isotachoforesy a kapilární zónové elektroforesy

Popis výsledku v původním jazyce

The aim of this work was to develop and validate electrophoretic methods for the determination of a new antiepileptic, lamotrigine (LAM). We tested the conditions for analysis of LAM in blood serum by two capillary isotachophoreses (ITP-ITP) and by capilary isotachophoresis - capillary zone electrophoresis (ITP-CZE). Best results were obtained using the ITP-CZE method with UV detection at 254 nm. The latter method offers a simple and rapid procedure for the determination of LAM in blood serum. A low sample volume is highly convenient especially for the determination in children. The analysis takes some 20 min. The linearity of the method was proved in the range 0-20 mg LAM/I. The limits of detection and quantification were 0.25 and 0.83 mg LAM/l, respectively. The variation coefficients were below 10% at three concentration levels (1, 7 and 20 mg l(-1)). The method was compared with HPLC using the Passing-Bablok regression analysis; no significant differences were found.

Název v anglickém jazyce

Determination of Lamotrigine by Isotachophoresis - Capillary Zone Electrophoresis

Popis výsledku anglicky

The aim of this work was to develop and validate electrophoretic methods for the determination of a new antiepileptic, lamotrigine (LAM). We tested the conditions for analysis of LAM in blood serum by two capillary isotachophoreses (ITP-ITP) and by capilary isotachophoresis - capillary zone electrophoresis (ITP-CZE). Best results were obtained using the ITP-CZE method with UV detection at 254 nm. The latter method offers a simple and rapid procedure for the determination of LAM in blood serum. A low sample volume is highly convenient especially for the determination in children. The analysis takes some 20 min. The linearity of the method was proved in the range 0-20 mg LAM/I. The limits of detection and quantification were 0.25 and 0.83 mg LAM/l, respectively. The variation coefficients were below 10% at three concentration levels (1, 7 and 20 mg l(-1)). The method was compared with HPLC using the Passing-Bablok regression analysis; no significant differences were found.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemické listy

ISSN

0009-2770

e-ISSN

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Svazek periodika

103

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

000263745600008

EID výsledku v databázi Scopus

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