ELISA estimation of S-equol in human body fluids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00024177" target="_blank" >RIV/60461373:22330/10:00024177 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

ELISA estimation of S-equol in human body fluids

Popis výsledku v původním jazyce

Equol is a biologically active metabolite of isoflavonoid daidzein, which is the principle of food supplements designated for women in climacterium. The conversion from daidzein to equol is caused by the intestinal microflora. Therefore this microflora differs bethween individuals, the production of equol isn?t uniform. Equol is a chiral molecule. Always S-equol rise by the action of intestinal microflora in human body, R-equol was prepared only synthetically. S-equol acts as a weak agonist in estrogenreceptors ? and therefore is in many cases a good alternative to hormone replacement therapy. The object of this study was to optimize the competitive enzyme-liked immunosorbent assay (ELISA) method for the determination of equol in human body fluids using enantiometrically pure standards. Racemic equol conjugates coupled to bovine serum albumine at positions 4'-O- via carboxymethyl spacer were used as immunogens. Conjugates of the same hapten with ovalbumine were used for coating of the

Název v anglickém jazyce

ELISA estimation of S-equol in human body fluids

Popis výsledku anglicky

Equol is a biologically active metabolite of isoflavonoid daidzein, which is the principle of food supplements designated for women in climacterium. The conversion from daidzein to equol is caused by the intestinal microflora. Therefore this microflora differs bethween individuals, the production of equol isn?t uniform. Equol is a chiral molecule. Always S-equol rise by the action of intestinal microflora in human body, R-equol was prepared only synthetically. S-equol acts as a weak agonist in estrogenreceptors ? and therefore is in many cases a good alternative to hormone replacement therapy. The object of this study was to optimize the competitive enzyme-liked immunosorbent assay (ELISA) method for the determination of equol in human body fluids using enantiometrically pure standards. Racemic equol conjugates coupled to bovine serum albumine at positions 4'-O- via carboxymethyl spacer were used as immunogens. Conjugates of the same hapten with ovalbumine were used for coating of the

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA303%2F08%2F0958" target="_blank" >GA303/08/0958: Fytoestrogeny jako alternativa hormonální substituční terapie - studium účinnosti a bezpečnosti</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Polyphenols Communications 2010

ISBN

978-2-7380-1282-1

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

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Název nakladatele

Groupe Polyphénols

Místo vydání

Montpellier

Místo konání akce

Montpellier, Francie

Datum konání akce

1. 1. 2010

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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