Classification of protein binders in artist's paints by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry: an evaluation of principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F11%3A43892137" target="_blank" >RIV/60461373:22330/11:43892137 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11410/11:10106853

Výsledek na webu

<a href="http://dx.doi.org/10.1002/rcm.5027" target="_blank" >http://dx.doi.org/10.1002/rcm.5027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/rcm.5027" target="_blank" >10.1002/rcm.5027</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Classification of protein binders in artist's paints by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry: an evaluation of principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA)

Popis výsledku v původním jazyce

Proteomics techniques are increasingly applied for the identification of protein binders in historical paints. The complex nature of paint samples, with different kinds of pigments mixed into, and degradation by long term exposure to light, humidity andtemperature variations, requires solid analysis and interpretation methods. In this study matrix?assisted laser desorption/ionisation time?of?flight (MALDI?TOF) mass spectra of tryptic?digested paint replicas are subjected to principal component analysis(PCA) and soft independent modelling of class analogy (SIMCA) in order to distinguish proteinaceous binders based on animal glues, egg white, egg yolk and milk casein from each other. The most meaningful peptide peaks for a given protein class will be determined, and if possible, annotated with their corresponding amino acid sequence. The methodology was subsequently applied on egg temperas, as well as on animal glues from different species. In the latter small differences in the MALDI-

Název v anglickém jazyce

Classification of protein binders in artist's paints by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry: an evaluation of principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA)

Popis výsledku anglicky

Proteomics techniques are increasingly applied for the identification of protein binders in historical paints. The complex nature of paint samples, with different kinds of pigments mixed into, and degradation by long term exposure to light, humidity andtemperature variations, requires solid analysis and interpretation methods. In this study matrix?assisted laser desorption/ionisation time?of?flight (MALDI?TOF) mass spectra of tryptic?digested paint replicas are subjected to principal component analysis(PCA) and soft independent modelling of class analogy (SIMCA) in order to distinguish proteinaceous binders based on animal glues, egg white, egg yolk and milk casein from each other. The most meaningful peptide peaks for a given protein class will be determined, and if possible, annotated with their corresponding amino acid sequence. The methodology was subsequently applied on egg temperas, as well as on animal glues from different species. In the latter small differences in the MALDI-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Rapid Communications in Mass Spectrometry

ISSN

0951-4198

e-ISSN

—

Svazek periodika

25

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1631-1640

Kód UT WoS článku

000290773800023

EID výsledku v databázi Scopus

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