Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes

Popis výsledku v původním jazyce

The aim of this work is the preparation of transgenic plant resistant to both organic and inorganic pollutants. Firstly transgenic plants containing bacterial bphC gene were prepared. The bphC gene is coding for 2,3- dihydroxybiphenyl-1,2-dioxygenase cleaving the biphenyl ring to form a product which could be further metabolized. The presence of bphC gene in first and second filial generation of transgenic plants was detected on the DNA and RNA level using PCR or RT-PCR methods. Transgenic plants contain the bphC gene in fusion with genes for detection markers GUS (gene for beta-glucuronidase) and LUC (gene for luciferase) and also bphC gene with histidine tail. The expression of the bphC/GUS and the bphC/LUC genes in plants was tested by histochemicalassay proving the expression of detection markers. Also the expression of the bphC/His gene was verified after purification of the enzyme by affinity chromatography followed by Western blot and immunochemical assay. The expression level

Název v anglickém jazyce

Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes

Popis výsledku anglicky

The aim of this work is the preparation of transgenic plant resistant to both organic and inorganic pollutants. Firstly transgenic plants containing bacterial bphC gene were prepared. The bphC gene is coding for 2,3- dihydroxybiphenyl-1,2-dioxygenase cleaving the biphenyl ring to form a product which could be further metabolized. The presence of bphC gene in first and second filial generation of transgenic plants was detected on the DNA and RNA level using PCR or RT-PCR methods. Transgenic plants contain the bphC gene in fusion with genes for detection markers GUS (gene for beta-glucuronidase) and LUC (gene for luciferase) and also bphC gene with histidine tail. The expression of the bphC/GUS and the bphC/LUC genes in plants was tested by histochemicalassay proving the expression of detection markers. Also the expression of the bphC/His gene was verified after purification of the enzyme by affinity chromatography followed by Western blot and immunochemical assay. The expression level

Druh

D - Stať ve sborníku

CEP obor

DK - Kontaminace a dekontaminace půdy včetně pesticidů

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

5th International Symposium on Biosorption and Bioremediation

ISBN

978-80-7080-825-2

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

118-121

Název nakladatele

VŠCHT Praha

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

24. 6. 2012

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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