Analysis of isoflavones in soybeans employing direct analysis in real-time ionization-high-resolution mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F12%3A43894790" target="_blank" >RIV/60461373:22330/12:43894790 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jssc.201100882" target="_blank" >http://dx.doi.org/10.1002/jssc.201100882</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201100882" target="_blank" >10.1002/jssc.201100882</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of isoflavones in soybeans employing direct analysis in real-time ionization-high-resolution mass spectrometry

Popis výsledku v původním jazyce

A direct analysis in real-time (DART) ion source coupled to a high-resolution orbitrap mass spectrometer was used for the quantitative analysis of isoflavones isolated from soybeans. For the isolation of genistein, daidzein, glycitein, and their respective acetyl, malonyl, and glucoside forms an extraction employing 80% aqueous MeOH enhanced by sonication was used. As far as the total isoflavones (expressed as aglycones) were to be determined, an acid hydrolysis with 80% aqueous EtOH and refluxing had to be employed, while in the latter case a good agreement of the results with the data generated by the UHPLC-orbitrap MS method was achieved, in the case of the analysis of nonhydrolyzed extracts, some overestimation of the results as compared with thosegenerated by UHPLC-orbitrap MS was observed. A careful investigation of this phenomenon showed that the free aglycones originated from the conjugated forms of isoflavones in the DART ion source, thus contributing significantly to the ''f

Název v anglickém jazyce

Analysis of isoflavones in soybeans employing direct analysis in real-time ionization-high-resolution mass spectrometry

Popis výsledku anglicky

A direct analysis in real-time (DART) ion source coupled to a high-resolution orbitrap mass spectrometer was used for the quantitative analysis of isoflavones isolated from soybeans. For the isolation of genistein, daidzein, glycitein, and their respective acetyl, malonyl, and glucoside forms an extraction employing 80% aqueous MeOH enhanced by sonication was used. As far as the total isoflavones (expressed as aglycones) were to be determined, an acid hydrolysis with 80% aqueous EtOH and refluxing had to be employed, while in the latter case a good agreement of the results with the data generated by the UHPLC-orbitrap MS method was achieved, in the case of the analysis of nonhydrolyzed extracts, some overestimation of the results as compared with thosegenerated by UHPLC-orbitrap MS was observed. A careful investigation of this phenomenon showed that the free aglycones originated from the conjugated forms of isoflavones in the DART ion source, thus contributing significantly to the ''f

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Svazek periodika

35

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

476-481

Kód UT WoS článku

000299254000018

EID výsledku v databázi Scopus

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