Effect of the Schiff base complex diaqua-(N-salicylidene-L-glutamato) copper(II) monohydrate on human tumor cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43895370" target="_blank" >RIV/60461373:22330/13:43895370 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0014299913006912" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0014299913006912</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejphar.2013.09.038" target="_blank" >10.1016/j.ejphar.2013.09.038</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of the Schiff base complex diaqua-(N-salicylidene-L-glutamato) copper(II) monohydrate on human tumor cells

Popis výsledku v původním jazyce

The aim of our study was to estimate cytostatic/cytotoxic activity of the copper(II) Schiff base complex of the composition [Cu(N-salicylidene-L-glutamato)(H2O)(2)]center dot H2O, further Cu(SG-L)H2O, against human colon carcinoma cell line HT-29, as well as to determine type of cell death and to find out the molecular mechanism of apoptosis induced by this complex. Two highest concentrations (50, 100 mu mol/l) of the complex showed a strong cytotoxic activity against human colon carcinoma cells HT-29 after 72 h of influence. Other concentrations had a cytostatic activity. Unchelated copper(II) ions and free ligands had no effect on the cell growth. Cu(SG-L)H2O preferentially reduced cancer cell viability compared to healthy cells (NIH-3T3). Cu(SG-L)H2O induced apoptosis of cells HT 29 at all concentrations used (1-100 mu mol/l) after 4811 of influence. Apoptosis was carried out by the mitochondrial pathway with active caspases 3 and 9. By the spin trapping technique combined with elec

Název v anglickém jazyce

Effect of the Schiff base complex diaqua-(N-salicylidene-L-glutamato) copper(II) monohydrate on human tumor cells

Popis výsledku anglicky

The aim of our study was to estimate cytostatic/cytotoxic activity of the copper(II) Schiff base complex of the composition [Cu(N-salicylidene-L-glutamato)(H2O)(2)]center dot H2O, further Cu(SG-L)H2O, against human colon carcinoma cell line HT-29, as well as to determine type of cell death and to find out the molecular mechanism of apoptosis induced by this complex. Two highest concentrations (50, 100 mu mol/l) of the complex showed a strong cytotoxic activity against human colon carcinoma cells HT-29 after 72 h of influence. Other concentrations had a cytostatic activity. Unchelated copper(II) ions and free ligands had no effect on the cell growth. Cu(SG-L)H2O preferentially reduced cancer cell viability compared to healthy cells (NIH-3T3). Cu(SG-L)H2O induced apoptosis of cells HT 29 at all concentrations used (1-100 mu mol/l) after 4811 of influence. Apoptosis was carried out by the mitochondrial pathway with active caspases 3 and 9. By the spin trapping technique combined with elec

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

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Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

EUROPEAN JOURNAL OF PHARMACOLOGY

ISSN

0014-2999

e-ISSN

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Svazek periodika

721

Číslo periodika v rámci svazku

1-3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

178-184

Kód UT WoS článku

000327541600024

EID výsledku v databázi Scopus

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