Immunodominant semen proteins I: New patterns of sperm proteins related to female immune infertility

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43895536" target="_blank" >RIV/60461373:22330/13:43895536 - isvavai.cz</a>

Výsledek na webu

<a href="http://download.springer.com/static/pdf/219/art%253A10.2478%252Fs11535-013-0214-9.pdf?auth66=1390735354_665cb5621b724b940a71486ef46585a4&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/219/art%253A10.2478%252Fs11535-013-0214-9.pdf?auth66=1390735354_665cb5621b724b940a71486ef46585a4&ext=.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11535-013-0214-9" target="_blank" >10.2478/s11535-013-0214-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunodominant semen proteins I: New patterns of sperm proteins related to female immune infertility

Popis výsledku v původním jazyce

Infertility affects approximately 10% of couples at reproductive age. Semen constituents may be potential immunogenic structures for women. The aim of our work is to detect and identify sperm proteins interacting with serum IgG antibodies from women withfertility disorders. The biochemical characterization of sperm antigens was performed using one and two dimensional gel electrophoresis, both of which were followed by immunoblotting. The IgG-binding proteins of interest were identified using mass spectrometry. From the serum pool of 30 infertile women, we detected sperm antigens within a relative molecular mass range between 30-80 kDa with an isoelectric point of 4-7. No antigens were detected using the serum pool from 10 fertile women (control group). Heat shock proteins (HSP 70) were identified as major sperm antigens associated with female immune infertility. Additionally, we report for the first time that alpha-enolase is a significant sperm antigen from the serum pool of infertil

Název v anglickém jazyce

Immunodominant semen proteins I: New patterns of sperm proteins related to female immune infertility

Popis výsledku anglicky

Infertility affects approximately 10% of couples at reproductive age. Semen constituents may be potential immunogenic structures for women. The aim of our work is to detect and identify sperm proteins interacting with serum IgG antibodies from women withfertility disorders. The biochemical characterization of sperm antigens was performed using one and two dimensional gel electrophoresis, both of which were followed by immunoblotting. The IgG-binding proteins of interest were identified using mass spectrometry. From the serum pool of 30 infertile women, we detected sperm antigens within a relative molecular mass range between 30-80 kDa with an isoelectric point of 4-7. No antigens were detected using the serum pool from 10 fertile women (control group). Heat shock proteins (HSP 70) were identified as major sperm antigens associated with female immune infertility. Additionally, we report for the first time that alpha-enolase is a significant sperm antigen from the serum pool of infertil

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

—

Projekt

<a href="/cs/project/IAA600110902" target="_blank" >IAA600110902: Vliv ingesce půd,antropogenně kontaminovaných těžkými kovy,na změny biochemických a fyziologických parametrů laboratorního potkana</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Central European Journal Of Biology

ISSN

1895-104X

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

6

Strana od-do

813-818

Kód UT WoS článku

000321069400001

EID výsledku v databázi Scopus

—