Development of antimicrobial packaging materials with immobilized glucose oxidase and lysozyme

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43895703" target="_blank" >RIV/60461373:22330/13:43895703 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22310/13:43895703 RIV/60461373:22810/13:43895703

Výsledek na webu

<a href="http://download.springer.com/static/pdf/208/art%253A10.2478%252Fs11532-013-0241-4.pdf?auth66=1391680283_bcbac8879eccecde1ab2f9a11b20a1ba&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/208/art%253A10.2478%252Fs11532-013-0241-4.pdf?auth66=1391680283_bcbac8879eccecde1ab2f9a11b20a1ba&ext=.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11532-013-0241-4" target="_blank" >10.2478/s11532-013-0241-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of antimicrobial packaging materials with immobilized glucose oxidase and lysozyme

Popis výsledku v původním jazyce

Packaging based on immobilization of antimicrobial enzymes provides a promising form of active packaging systems applicable in food processing. Glucose oxidase and lysozyme were immobilized by the Ugi reaction with cyclohexyl isocyanide and glutaraldehyde on polyamide and ionomer films partially hydrolysed by hydrochloric acid. The immobilization of the enzymes on the surface of films was confirmed by FT-IR spectroscopy and the films were characterized by the specific activity of the immobilized enzymes. The enzyme migration into model solutions and the effect of pH, temperature and storage time on the activity of immobilized enzyme were also evaluated. Immobilization of lysozyme onto polyamide and ionomer films resulted in the loss of enzyme activity.The polyamide and ionomer films with immobilized glucose oxidase inhibited the growth of bacteria Escherichia coli CNCTC 6859, Pseudomonas fluorescens CNCTC 5793, Lactobacillus helveticus CH-1, Listeria ivanovii CCM 5884 and Listeria inn

Název v anglickém jazyce

Development of antimicrobial packaging materials with immobilized glucose oxidase and lysozyme

Popis výsledku anglicky

Packaging based on immobilization of antimicrobial enzymes provides a promising form of active packaging systems applicable in food processing. Glucose oxidase and lysozyme were immobilized by the Ugi reaction with cyclohexyl isocyanide and glutaraldehyde on polyamide and ionomer films partially hydrolysed by hydrochloric acid. The immobilization of the enzymes on the surface of films was confirmed by FT-IR spectroscopy and the films were characterized by the specific activity of the immobilized enzymes. The enzyme migration into model solutions and the effect of pH, temperature and storage time on the activity of immobilized enzyme were also evaluated. Immobilization of lysozyme onto polyamide and ionomer films resulted in the loss of enzyme activity.The polyamide and ionomer films with immobilized glucose oxidase inhibited the growth of bacteria Escherichia coli CNCTC 6859, Pseudomonas fluorescens CNCTC 5793, Lactobacillus helveticus CH-1, Listeria ivanovii CCM 5884 and Listeria inn

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Central European Journal of Chemistry

ISSN

1895-1066

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

1066-1078

Kód UT WoS článku

000318180300005

EID výsledku v databázi Scopus

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