Rapid Immunoassays for Detection of Anabolic Nortestosterone in Dietary Supplements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43896619" target="_blank" >RIV/60461373:22330/13:43896619 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.agriculturejournals.cz/publicFiles/100650.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/100650.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Rapid Immunoassays for Detection of Anabolic Nortestosterone in Dietary Supplements

Popis výsledku v původním jazyce

An enzyme immunoassay (ELISA) and an immunochromatographic strip were designed for a rapid detection of nortestosterone in dietary supplements. Two polyclonal antibodies and two types of nortestosterone-protein coating conjugates were tested to develop the most appropriate method. Under optimal experimental conditions, the most sensitive ELISA achieved the IC50 and the limit of detection values of 6.41 and 0.09 ng/mL, respectively. Assay specificity was tested measuring cross-reactivity of several steroids. The interference to the assay was negligible ({0.1%), expect for cross-reactivity with another frequently abused steroid testosterone (23%). The optimized gold particle-based immunochromatographic strip provided in semi-quantitative test a visual detection limit of 1 ng/mL. None of these methods shows the interferences using a filtrate from suspension of non-contaminated sample. After validation for particular matrices, the ELISA and the strip test could be useful tools for rapid an

Název v anglickém jazyce

Rapid Immunoassays for Detection of Anabolic Nortestosterone in Dietary Supplements

Popis výsledku anglicky

An enzyme immunoassay (ELISA) and an immunochromatographic strip were designed for a rapid detection of nortestosterone in dietary supplements. Two polyclonal antibodies and two types of nortestosterone-protein coating conjugates were tested to develop the most appropriate method. Under optimal experimental conditions, the most sensitive ELISA achieved the IC50 and the limit of detection values of 6.41 and 0.09 ng/mL, respectively. Assay specificity was tested measuring cross-reactivity of several steroids. The interference to the assay was negligible ({0.1%), expect for cross-reactivity with another frequently abused steroid testosterone (23%). The optimized gold particle-based immunochromatographic strip provided in semi-quantitative test a visual detection limit of 1 ng/mL. None of these methods shows the interferences using a filtrate from suspension of non-contaminated sample. After validation for particular matrices, the ELISA and the strip test could be useful tools for rapid an

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/VG20112015045" target="_blank" >VG20112015045: Vývoj rychlých imunochemických testů pro detekci anabolicky aktivních steroidů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Food Sciences

ISSN

1212-1800

e-ISSN

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Svazek periodika

31

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

514-519

Kód UT WoS článku

000325509000013

EID výsledku v databázi Scopus

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