Rapid immunoassay for the detection of anabolic steroid methandienone
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43899085" target="_blank" >RIV/60461373:22330/14:43899085 - isvavai.cz</a>
Výsledek na webu
—
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Rapid immunoassay for the detection of anabolic steroid methandienone
Popis výsledku v původním jazyce
Methandienone, sometimes known as Dianabol is one of the most commonly abused anabolic steroid. The aim of this work is to develop an indirect competitive Enzyme Linked ImmunoSorbent Assay (ELISA) for the detection of methandienone in food supplements. Under the optimal experimental conditions, the most sensitive assay achieved IC50 and the limit of the detection the values of 1.45 ng/mL and 0.17 ng/mL, respectively. Specificity was verified by the testing of the cross-reactivity. The reactivity was examined by 44 standards of anabolic steroids. Furthermore, the different matrices of dietary supplements and sample preparation were tested and finally the method was successfully applied to real samples.
Název v anglickém jazyce
Rapid immunoassay for the detection of anabolic steroid methandienone
Popis výsledku anglicky
Methandienone, sometimes known as Dianabol is one of the most commonly abused anabolic steroid. The aim of this work is to develop an indirect competitive Enzyme Linked ImmunoSorbent Assay (ELISA) for the detection of methandienone in food supplements. Under the optimal experimental conditions, the most sensitive assay achieved IC50 and the limit of the detection the values of 1.45 ng/mL and 0.17 ng/mL, respectively. Specificity was verified by the testing of the cross-reactivity. The reactivity was examined by 44 standards of anabolic steroids. Furthermore, the different matrices of dietary supplements and sample preparation were tested and finally the method was successfully applied to real samples.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
GM - Potravinářství
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/VG20112015045" target="_blank" >VG20112015045: Vývoj rychlých imunochemických testů pro detekci anabolicky aktivních steroidů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů