Development of immunochemical method for the methandienone detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43899088" target="_blank" >RIV/60461373:22330/14:43899088 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of immunochemical method for the methandienone detection

Popis výsledku v původním jazyce

Methandienone, 17beta-hydroxy-17alfa-methylandrosta-1,4-dien-3-one, also known as DianabolTM is a synthetic exogenous steroid derived from testosterone. The aim of this work was to develop two formats of immunochromatographic test for the detection of anabolic steroid methandienone in nutritional supplements based on methandienone haptens which were conjugated in position C3 and C17 of steroid skeleton. Suitable conditions (concentration of immunoreagents, type of membrane and reaction buffer) were selected, six points calibration curves were prepared and visual detection limits were obtained (1 mýg/mL and 5 mýg/mL for MD-3-CMO-OVA and for the MD-17-HS-OVA system, respectively). In real samples of food supplements, both systems successful detected methandienone and the similar steroid.

Název v anglickém jazyce

Development of immunochemical method for the methandienone detection

Popis výsledku anglicky

Methandienone, 17beta-hydroxy-17alfa-methylandrosta-1,4-dien-3-one, also known as DianabolTM is a synthetic exogenous steroid derived from testosterone. The aim of this work was to develop two formats of immunochromatographic test for the detection of anabolic steroid methandienone in nutritional supplements based on methandienone haptens which were conjugated in position C3 and C17 of steroid skeleton. Suitable conditions (concentration of immunoreagents, type of membrane and reaction buffer) were selected, six points calibration curves were prepared and visual detection limits were obtained (1 mýg/mL and 5 mýg/mL for MD-3-CMO-OVA and for the MD-17-HS-OVA system, respectively). In real samples of food supplements, both systems successful detected methandienone and the similar steroid.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/VG20112015045" target="_blank" >VG20112015045: Vývoj rychlých imunochemických testů pro detekci anabolicky aktivních steroidů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů