DETERMINATION OF FLAVONOIDS IN STELLARIA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897206" target="_blank" >RIV/60461373:22330/14:43897206 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00023761:_____/14:#0000468
Výsledek na webu
<a href="http://dx.doi.org/10.1080/00032719.2014.908382" target="_blank" >http://dx.doi.org/10.1080/00032719.2014.908382</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00032719.2014.908382" target="_blank" >10.1080/00032719.2014.908382</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
DETERMINATION OF FLAVONOIDS IN STELLARIA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY
Popis výsledku v původním jazyce
genus Stellaria (Caryophyllaceae) presents widely distributed plants often used in traditional medicine. Flavonoids are highly active plant secondary metabolites that may be involved in some of the effects of Stellaria plants. In this study, two new high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS-MS) methods were developed for the determination of flavonoids and isoflavonoids in plant material. The separations were performed on a reverse-phase C18 column with gradient elution using methanol and water with 0.5% acetic acid as the mobile phase. Multiple reaction monitoring was used for the tandem mass spectrometry detection and the two most intensive transitions were chosen for the identification of each analyte. The limits of detection and the limits of quantification were between 0.2 and 15.0 ng/mL and 0.6 and 50.0 ng/mL. The developed methods were successfully used for the analyses of four representatives of the genus Stellaria. All
Název v anglickém jazyce
DETERMINATION OF FLAVONOIDS IN STELLARIA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY
Popis výsledku anglicky
genus Stellaria (Caryophyllaceae) presents widely distributed plants often used in traditional medicine. Flavonoids are highly active plant secondary metabolites that may be involved in some of the effects of Stellaria plants. In this study, two new high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS-MS) methods were developed for the determination of flavonoids and isoflavonoids in plant material. The separations were performed on a reverse-phase C18 column with gradient elution using methanol and water with 0.5% acetic acid as the mobile phase. Multiple reaction monitoring was used for the tandem mass spectrometry detection and the two most intensive transitions were chosen for the identification of each analyte. The limits of detection and the limits of quantification were between 0.2 and 15.0 ng/mL and 0.6 and 50.0 ng/mL. The developed methods were successfully used for the analyses of four representatives of the genus Stellaria. All
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA525%2F09%2F0994" target="_blank" >GA525/09/0994: Chemotaxonomie isoflavonoidů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
ANALYTICAL LETTERS
ISSN
0003-2719
e-ISSN
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Svazek periodika
47
Číslo periodika v rámci svazku
14
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
15
Strana od-do
2317-2331
Kód UT WoS článku
000340474500002
EID výsledku v databázi Scopus
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