Compression tests of a living cell: a contact detection problem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897522" target="_blank" >RIV/60461373:22330/14:43897522 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.tandfonline.com/doi/abs/10.1080/10255842.2014.931093?journalCode=gcmb20#.VLaWhytfZ8F" target="_blank" >http://www.tandfonline.com/doi/abs/10.1080/10255842.2014.931093?journalCode=gcmb20#.VLaWhytfZ8F</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/10255842.2014.931093" target="_blank" >10.1080/10255842.2014.931093</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Compression tests of a living cell: a contact detection problem

Popis výsledku v původním jazyce

Alterations in the mechanical properties of cells are associated with cellular diversification and diseases. Assessment of mechanical properties of living cells was mostly associated with Atomic Force Microscopy technique. However, the contact conditionsof AFM tip with cell's membrane are very complex (Vichare et al. 2012). Advantageously, very well defined geometry of flat end probe used for nanoindentation can simplify the contact problem into compression of an inflated spherical membrane by two rigid parallel planes (Nadler 2010 and Arfsten et al. 2008). From our previous experiments we used COS-1 cells [ATCC, USA] because of their long viability and good adhesion properties. This cell line [ATCC code: CRL-1650] was derived from African green monkey kidney; the cells grow attached to the base (adherent) and have morphology of fibroblasts. The aims of present study are as follows: (1) Determination of the contact between flat punch tip and single cell and (2) to identify membrane bu

Název v anglickém jazyce

Compression tests of a living cell: a contact detection problem

Popis výsledku anglicky

Alterations in the mechanical properties of cells are associated with cellular diversification and diseases. Assessment of mechanical properties of living cells was mostly associated with Atomic Force Microscopy technique. However, the contact conditionsof AFM tip with cell's membrane are very complex (Vichare et al. 2012). Advantageously, very well defined geometry of flat end probe used for nanoindentation can simplify the contact problem into compression of an inflated spherical membrane by two rigid parallel planes (Nadler 2010 and Arfsten et al. 2008). From our previous experiments we used COS-1 cells [ATCC, USA] because of their long viability and good adhesion properties. This cell line [ATCC code: CRL-1650] was derived from African green monkey kidney; the cells grow attached to the base (adherent) and have morphology of fibroblasts. The aims of present study are as follows: (1) Determination of the contact between flat punch tip and single cell and (2) to identify membrane bu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JD - Využití počítačů, robotika a její aplikace

OECD FORD obor

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Projekt

<a href="/cs/project/LH12011" target="_blank" >LH12011: Struktura retrovirové částice a intracelulární transport jejích komponent</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Computer Methods in Biomechanics and Biomedical Engineering

ISSN

1025-5842

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

30.7.2014

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

2

Strana od-do

40-41

Kód UT WoS článku

000340376700021

EID výsledku v databázi Scopus

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