Compression tests of a living cell: a contact detection problem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F14%3A00217609" target="_blank" >RIV/68407700:21220/14:00217609 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/10255842.2014.931093" target="_blank" >http://dx.doi.org/10.1080/10255842.2014.931093</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/10255842.2014.931093" target="_blank" >10.1080/10255842.2014.931093</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Compression tests of a living cell: a contact detection problem

Popis výsledku v původním jazyce

Alterations in the mechanical properties of cells are associated with cellular diversification and diseases. Assessment of mechanical properties of living cells was mostly associated with Atomic Force Microscopy technique. However, the contact conditions of AFM tip with cell’s membrane are very complex. Advantageously, very well defined geometry of flat end probe used for nanoindentation can simplify the contact problem into compression of an inflated spherical membrane by two rigid parallel planes. From our previous experiments we used COS-1 cells [ATCC, USA] because of their long viability and good adhesion properties. This cell line [ATCC code: CRL-1650] was derived from African green monkey kidney; the cells grow attached to the base (adherent) and have morphology of fibroblasts. The aims of present study are as follows: (1) Determination of the contact between flat punch tip and single cell and (2) to identify membrane bursting critical load.

Název v anglickém jazyce

Compression tests of a living cell: a contact detection problem

Popis výsledku anglicky

Alterations in the mechanical properties of cells are associated with cellular diversification and diseases. Assessment of mechanical properties of living cells was mostly associated with Atomic Force Microscopy technique. However, the contact conditions of AFM tip with cell’s membrane are very complex. Advantageously, very well defined geometry of flat end probe used for nanoindentation can simplify the contact problem into compression of an inflated spherical membrane by two rigid parallel planes. From our previous experiments we used COS-1 cells [ATCC, USA] because of their long viability and good adhesion properties. This cell line [ATCC code: CRL-1650] was derived from African green monkey kidney; the cells grow attached to the base (adherent) and have morphology of fibroblasts. The aims of present study are as follows: (1) Determination of the contact between flat punch tip and single cell and (2) to identify membrane bursting critical load.

Druh

D - Stať ve sborníku

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/TA01010185" target="_blank" >TA01010185: Nové materiály a povrchové vrstvy pro bionický návrh kloubních náhrad</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Computer Methods in Biomechanics and Biomedical Engineering

ISBN

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ISSN

1025-5842

e-ISSN

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Počet stran výsledku

2

Strana od-do

40-41

Název nakladatele

Taylor & Francis

Místo vydání

London

Místo konání akce

Valenciennes

Datum konání akce

27. 8. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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